Results 1 - 20 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12678 | 3' | -52.4 | NC_003345.1 | + | 2138 | 0.66 | 0.903939 |
Target: 5'- cGGCGAGAGugacaUCGCuaAGUgGUGcgaagCGCCc -3' miRNA: 3'- aUCGCUCUC-----AGCG--UCAgCAUua---GCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 9479 | 0.66 | 0.929197 |
Target: 5'- gAGCGucggcuuggucuuccGGGUCGUGGUaGUAucgGUCGCCa -3' miRNA: 3'- aUCGCu--------------CUCAGCGUCAgCAU---UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 10260 | 0.7 | 0.745582 |
Target: 5'- ---aGGGAGUagaaGUAGUCGUAGUCGgCu -3' miRNA: 3'- aucgCUCUCAg---CGUCAGCAUUAGCgG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 10911 | 0.66 | 0.928636 |
Target: 5'- cGGgGAGGGa-GCAGUCcuuacaGUuGUCGCCg -3' miRNA: 3'- aUCgCUCUCagCGUCAG------CAuUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 11006 | 0.66 | 0.897086 |
Target: 5'- -cGUGAGAGcCGCuauGUCGgcguucAUCGCUc -3' miRNA: 3'- auCGCUCUCaGCGu--CAGCau----UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 12403 | 0.69 | 0.805498 |
Target: 5'- gAGUGGGAGUCGCuGaCGgagGAcaaCGCCa -3' miRNA: 3'- aUCGCUCUCAGCGuCaGCa--UUa--GCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 12588 | 0.67 | 0.858935 |
Target: 5'- cGGCucGGGAGUaCGCu-UCGUAgagGUCGCCg -3' miRNA: 3'- aUCG--CUCUCA-GCGucAGCAU---UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 14636 | 0.66 | 0.928636 |
Target: 5'- aUAGUcGGGGUCGCAcGgccuccacUCGUGGUCGUg -3' miRNA: 3'- -AUCGcUCUCAGCGU-C--------AGCAUUAGCGg -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 15328 | 0.7 | 0.703199 |
Target: 5'- uUGGUGAG-GUCGgGGccgaCGUGGUUGCCg -3' miRNA: 3'- -AUCGCUCuCAGCgUCa---GCAUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 19940 | 0.73 | 0.550543 |
Target: 5'- -cGUGGuAGUCGCGGUCGUcGUCGUUa -3' miRNA: 3'- auCGCUcUCAGCGUCAGCAuUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 20466 | 0.73 | 0.550543 |
Target: 5'- gGGUucGAGAGUCGCu-UCGaGGUCGCCg -3' miRNA: 3'- aUCG--CUCUCAGCGucAGCaUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 21767 | 0.66 | 0.922873 |
Target: 5'- -cGUcGGAGUCaCcGUCGUcGUCGCCg -3' miRNA: 3'- auCGcUCUCAGcGuCAGCAuUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 32769 | 0.66 | 0.903939 |
Target: 5'- -uGCGAGAGa-GCGGUCGgcGUUGaCa -3' miRNA: 3'- auCGCUCUCagCGUCAGCauUAGCgG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 34397 | 0.69 | 0.795902 |
Target: 5'- cGGUGAGcuGGUCGCugaGGUCGUccAGUUGCg -3' miRNA: 3'- aUCGCUC--UCAGCG---UCAGCA--UUAGCGg -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 34410 | 0.66 | 0.928636 |
Target: 5'- gAGgGAcAGUCGCAGacCGgacUCGCCg -3' miRNA: 3'- aUCgCUcUCAGCGUCa-GCauuAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 36004 | 0.67 | 0.882587 |
Target: 5'- gGGaaGGAGUCGCcccGGUC-UGGUCGCUg -3' miRNA: 3'- aUCgcUCUCAGCG---UCAGcAUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 39325 | 0.71 | 0.648747 |
Target: 5'- cGGUGAGAGaaucccgaauuUCGuUAGUCGUGuaccacccGUCGCCg -3' miRNA: 3'- aUCGCUCUC-----------AGC-GUCAGCAU--------UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 41219 | 0.67 | 0.880323 |
Target: 5'- cGGCGAGguuucggucuucguAGUCuCGGUCGaGGUCGUCc -3' miRNA: 3'- aUCGCUC--------------UCAGcGUCAGCaUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 41676 | 0.7 | 0.703199 |
Target: 5'- aAGCGGGAGggcCGUAGUag-AAUCGUCg -3' miRNA: 3'- aUCGCUCUCa--GCGUCAgcaUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 42955 | 0.68 | 0.833163 |
Target: 5'- aUAGUGA-AGUCGCGGUUGUccucuuUCGCa -3' miRNA: 3'- -AUCGCUcUCAGCGUCAGCAuu----AGCGg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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