Results 21 - 32 of 32 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12678 | 3' | -52.4 | NC_003345.1 | + | 41219 | 0.67 | 0.880323 |
Target: 5'- cGGCGAGguuucggucuucguAGUCuCGGUCGaGGUCGUCc -3' miRNA: 3'- aUCGCUC--------------UCAGcGUCAGCaUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 36004 | 0.67 | 0.882587 |
Target: 5'- gGGaaGGAGUCGCcccGGUC-UGGUCGCUg -3' miRNA: 3'- aUCgcUCUCAGCG---UCAGcAUUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 63117 | 0.67 | 0.882587 |
Target: 5'- cAGcCGAGuAGUCgGCAGUagCGUAguccaccgaGUCGCCc -3' miRNA: 3'- aUC-GCUC-UCAG-CGUCA--GCAU---------UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 11006 | 0.66 | 0.897086 |
Target: 5'- -cGUGAGAGcCGCuauGUCGgcguucAUCGCUc -3' miRNA: 3'- auCGCUCUCaGCGu--CAGCau----UAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 32769 | 0.66 | 0.903939 |
Target: 5'- -uGCGAGAGa-GCGGUCGgcGUUGaCa -3' miRNA: 3'- auCGCUCUCagCGUCAGCauUAGCgG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 50324 | 0.66 | 0.903939 |
Target: 5'- --uCGGGGGUCGCGGUgGUAAUgGa- -3' miRNA: 3'- aucGCUCUCAGCGUCAgCAUUAgCgg -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 2138 | 0.66 | 0.903939 |
Target: 5'- cGGCGAGAGugacaUCGCuaAGUgGUGcgaagCGCCc -3' miRNA: 3'- aUCGCUCUC-----AGCG--UCAgCAUua---GCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 21767 | 0.66 | 0.922873 |
Target: 5'- -cGUcGGAGUCaCcGUCGUcGUCGCCg -3' miRNA: 3'- auCGcUCUCAGcGuCAGCAuUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 10911 | 0.66 | 0.928636 |
Target: 5'- cGGgGAGGGa-GCAGUCcuuacaGUuGUCGCCg -3' miRNA: 3'- aUCgCUCUCagCGUCAG------CAuUAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 14636 | 0.66 | 0.928636 |
Target: 5'- aUAGUcGGGGUCGCAcGgccuccacUCGUGGUCGUg -3' miRNA: 3'- -AUCGcUCUCAGCGU-C--------AGCAUUAGCGg -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 34410 | 0.66 | 0.928636 |
Target: 5'- gAGgGAcAGUCGCAGacCGgacUCGCCg -3' miRNA: 3'- aUCgCUcUCAGCGUCa-GCauuAGCGG- -5' |
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12678 | 3' | -52.4 | NC_003345.1 | + | 9479 | 0.66 | 0.929197 |
Target: 5'- gAGCGucggcuuggucuuccGGGUCGUGGUaGUAucgGUCGCCa -3' miRNA: 3'- aUCGCu--------------CUCAGCGUCAgCAU---UAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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