Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12683 | 3' | -61.8 | NC_003345.1 | + | 21483 | 0.69 | 0.349467 |
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Target: 5'- uGGCUGAACgaggaCGCucucgaacuccgugaCGACCUCGaaGGCCgCCg -3' miRNA: 3'- -CCGACUUG-----GCG---------------GCUGGAGCg-CCGG-GG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 70173 | 0.68 | 0.394685 |
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Target: 5'- -cCUGAACCuCCGAga-CGCGGCCCUc -3' miRNA: 3'- ccGACUUGGcGGCUggaGCGCCGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 33713 | 0.68 | 0.411727 |
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Target: 5'- aGCUGAACaacgagguguaCGuuGACCUCGCcGCCg- -3' miRNA: 3'- cCGACUUG-----------GCggCUGGAGCGcCGGgg -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 75640 | 0.67 | 0.429217 |
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Target: 5'- aGCUGuuCCGUCG-CgCUCGCGGUCUUc -3' miRNA: 3'- cCGACuuGGCGGCuG-GAGCGCCGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 24389 | 0.67 | 0.456251 |
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Target: 5'- gGGUUGAcguugACuCGuCCGACCUCGCucGUCUCg -3' miRNA: 3'- -CCGACU-----UG-GC-GGCUGGAGCGc-CGGGG- -5' |
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| 12683 | 3' | -61.8 | NC_003345.1 | + | 75304 | 1.13 | 0.000229 |
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Target: 5'- gGGCUGAACCGCCGACCUCGCGGCCCCg -3' miRNA: 3'- -CCGACUUGGCGGCUGGAGCGCCGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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