Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12702 | 3' | -50.4 | NC_003349.1 | + | 113637 | 0.68 | 0.971462 |
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Target: 5'- uAGCAACagucgcggacgcaGACGCaGCCGAAGcCGUaGCc -3' miRNA: 3'- -UCGUUGg------------CUGCGaUGGCUUUuGCA-CG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 113677 | 0.67 | 0.987839 |
|
Target: 5'- cGCAGCCGuacuUGUcACCGAcGACGccGCa -3' miRNA: 3'- uCGUUGGCu---GCGaUGGCUuUUGCa-CG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 116630 | 0.76 | 0.675525 |
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Target: 5'- cAGCAacguuguuACCGAUcaGC-GCCGAAAAUGUGCg -3' miRNA: 3'- -UCGU--------UGGCUG--CGaUGGCUUUUGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 116761 | 0.74 | 0.806422 |
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Target: 5'- uGCAGCCGccacCGCcGCCGAAGACGaUGa -3' miRNA: 3'- uCGUUGGCu---GCGaUGGCUUUUGC-ACg -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 128736 | 0.69 | 0.963034 |
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Target: 5'- gAGUGAUgGACGCggacgaUGCCGAAGAauUGCa -3' miRNA: 3'- -UCGUUGgCUGCG------AUGGCUUUUgcACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 128862 | 0.7 | 0.932178 |
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Target: 5'- uAGUAcaaAUCGAUGCUaACCGAAAAUG-GCc -3' miRNA: 3'- -UCGU---UGGCUGCGA-UGGCUUUUGCaCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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