Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12702 | 3' | -50.4 | NC_003349.1 | + | 57541 | 0.67 | 0.984402 |
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Target: 5'- cAGUAgaACCGGCugacgaaacuGCUGCUGuugagaguAAACGUGCu -3' miRNA: 3'- -UCGU--UGGCUG----------CGAUGGCu-------UUUGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 113677 | 0.67 | 0.987839 |
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Target: 5'- cGCAGCCGuacuUGUcACCGAcGACGccGCa -3' miRNA: 3'- uCGUUGGCu---GCGaUGGCUuUUGCa-CG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 94111 | 0.67 | 0.989322 |
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Target: 5'- -uCGACCGuuuaGCGUgACCGAuuguGAAUGUGCu -3' miRNA: 3'- ucGUUGGC----UGCGaUGGCU----UUUGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 51544 | 0.66 | 0.990532 |
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Target: 5'- uGGCAACgGACGCU-CCGGugggaaucgccauGGGuaugaacgcCGUGCa -3' miRNA: 3'- -UCGUUGgCUGCGAuGGCU-------------UUU---------GCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 58887 | 0.66 | 0.993897 |
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Target: 5'- cGGCGugacacccGCCGAuguuguCGUUGCCGGu--UGUGCg -3' miRNA: 3'- -UCGU--------UGGCU------GCGAUGGCUuuuGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 76258 | 0.66 | 0.994749 |
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Target: 5'- cGUAACCGucccuaggauuACGUuuaUACaUGAGAAUGUGCg -3' miRNA: 3'- uCGUUGGC-----------UGCG---AUG-GCUUUUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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