Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12702 | 3' | -50.4 | NC_003349.1 | + | 59132 | 0.7 | 0.926746 |
Target: 5'- cGCAGCCGACGCaACCaucuGCGccGCc -3' miRNA: 3'- uCGUUGGCUGCGaUGGcuuuUGCa-CG- -5' |
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12702 | 3' | -50.4 | NC_003349.1 | + | 104104 | 0.71 | 0.908886 |
Target: 5'- uGCGACUG-CGaaACCGGuuACGUGCc -3' miRNA: 3'- uCGUUGGCuGCgaUGGCUuuUGCACG- -5' |
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12702 | 3' | -50.4 | NC_003349.1 | + | 22002 | 0.72 | 0.888718 |
Target: 5'- gAGUAAuCCGugGCUACgGGuaaaguaguAAUGUGCa -3' miRNA: 3'- -UCGUU-GGCugCGAUGgCUu--------UUGCACG- -5' |
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12702 | 3' | -50.4 | NC_003349.1 | + | 116761 | 0.74 | 0.806422 |
Target: 5'- uGCAGCCGccacCGCcGCCGAAGACGaUGa -3' miRNA: 3'- uCGUUGGCu---GCGaUGGCUUUUGC-ACg -5' |
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12702 | 3' | -50.4 | NC_003349.1 | + | 116630 | 0.76 | 0.675525 |
Target: 5'- cAGCAacguuguuACCGAUcaGC-GCCGAAAAUGUGCg -3' miRNA: 3'- -UCGU--------UGGCUG--CGaUGGCUUUUGCACG- -5' |
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12702 | 3' | -50.4 | NC_003349.1 | + | 73661 | 1.13 | 0.0052 |
Target: 5'- gAGCAACCGACGCUACCGAAAACGUGCu -3' miRNA: 3'- -UCGUUGGCUGCGAUGGCUUUUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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