Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12702 | 3' | -50.4 | NC_003349.1 | + | 99931 | 0.69 | 0.962678 |
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Target: 5'- uGGCAACCGAauaCGCUuuucaucACCGAAGcCG-GUa -3' miRNA: 3'- -UCGUUGGCU---GCGA-------UGGCUUUuGCaCG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 128736 | 0.69 | 0.963034 |
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Target: 5'- gAGUGAUgGACGCggacgaUGCCGAAGAauUGCa -3' miRNA: 3'- -UCGUUGgCUGCG------AUGGCUUUUgcACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 99115 | 0.68 | 0.975379 |
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Target: 5'- gAGguGCuCGACGaaACUGAAuuACGUGCc -3' miRNA: 3'- -UCguUG-GCUGCgaUGGCUUu-UGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 59391 | 0.68 | 0.975379 |
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Target: 5'- uGCAaaaGCUugGACGCUACUGAAGAUauuuUGCa -3' miRNA: 3'- uCGU---UGG--CUGCGAUGGCUUUUGc---ACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 57541 | 0.67 | 0.984402 |
|
Target: 5'- cAGUAgaACCGGCugacgaaacuGCUGCUGuugagaguAAACGUGCu -3' miRNA: 3'- -UCGU--UGGCUG----------CGAUGGCu-------UUUGCACG- -5' |
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| 12702 | 3' | -50.4 | NC_003349.1 | + | 73661 | 1.13 | 0.0052 |
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Target: 5'- gAGCAACCGACGCUACCGAAAACGUGCu -3' miRNA: 3'- -UCGUUGGCUGCGAUGGCUUUUGCACG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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