Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12781 | 3' | -54.5 | NC_003387.1 | + | 42914 | 0.66 | 0.754961 |
Target: 5'- aGCGCAGCcugcagcgccGGUcGCUCgacaaCAUGgucgcGCUCGCc -3' miRNA: 3'- -CGCGUCG----------UCAaCGAGg----GUAC-----UGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 24816 | 0.66 | 0.744451 |
Target: 5'- gGCGCAGCAGcucgGCguaCUCGggcGGCUgGCc -3' miRNA: 3'- -CGCGUCGUCaa--CGa--GGGUa--CUGAgCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 5289 | 0.66 | 0.744451 |
Target: 5'- aCGUGGguGUUGUgcaugaggcccUCCUcgGGCUCGg -3' miRNA: 3'- cGCGUCguCAACG-----------AGGGuaCUGAGCg -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 19296 | 0.66 | 0.733823 |
Target: 5'- cGCGCAGCAGcagGCacagCCgCAcaacgGcCUCGCg -3' miRNA: 3'- -CGCGUCGUCaa-CGa---GG-GUa----CuGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 33200 | 0.66 | 0.733823 |
Target: 5'- gGCaGCAGCgAGUUGC-CCCGguUGGCauccaCGCc -3' miRNA: 3'- -CG-CGUCG-UCAACGaGGGU--ACUGa----GCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 23969 | 0.66 | 0.733823 |
Target: 5'- uGCGCAGCgccgggcggcuGGUUGUgcaCCGccuGCUCGCu -3' miRNA: 3'- -CGCGUCG-----------UCAACGag-GGUac-UGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 16833 | 0.66 | 0.733823 |
Target: 5'- cCGCAGCAGgucgacgUGC-CCCuUGACgcUCGa -3' miRNA: 3'- cGCGUCGUCa------ACGaGGGuACUG--AGCg -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 31849 | 0.66 | 0.723091 |
Target: 5'- gGCGCGaCGG-UGCUgCUAUGACcggCGCc -3' miRNA: 3'- -CGCGUcGUCaACGAgGGUACUGa--GCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 36595 | 0.66 | 0.712266 |
Target: 5'- -gGCGGCAGUaa--CCCGgcguucGACUCGCg -3' miRNA: 3'- cgCGUCGUCAacgaGGGUa-----CUGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 10460 | 0.66 | 0.70136 |
Target: 5'- -aGCuGCGGcgUGUUCCCAgGugUCGg -3' miRNA: 3'- cgCGuCGUCa-ACGAGGGUaCugAGCg -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 46225 | 0.67 | 0.690385 |
Target: 5'- uGCuGCAGCAGgaggGCUCUCA--GC-CGCu -3' miRNA: 3'- -CG-CGUCGUCaa--CGAGGGUacUGaGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 8782 | 0.67 | 0.690385 |
Target: 5'- cGUGCGGCGGcggGCgaccugauUCCCGcucaGCUCGCg -3' miRNA: 3'- -CGCGUCGUCaa-CG--------AGGGUac--UGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 50286 | 0.67 | 0.690385 |
Target: 5'- cGCGCGuccauGCGGUUGCgcugccacaucUCCCAggcgGGgUCGUu -3' miRNA: 3'- -CGCGU-----CGUCAACG-----------AGGGUa---CUgAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 15393 | 0.67 | 0.679352 |
Target: 5'- cCGCGGCGGggGCUCguCCAcGGCacgucggCGCg -3' miRNA: 3'- cGCGUCGUCaaCGAG--GGUaCUGa------GCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 5326 | 0.67 | 0.668273 |
Target: 5'- gGCGgGGcCGGUUGC-CCCGc-GCUUGCg -3' miRNA: 3'- -CGCgUC-GUCAACGaGGGUacUGAGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 46651 | 0.67 | 0.657159 |
Target: 5'- aCGCAGaCAGgacggUGCUgCCCugcuUGGC-CGCg -3' miRNA: 3'- cGCGUC-GUCa----ACGA-GGGu---ACUGaGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 23815 | 0.67 | 0.657159 |
Target: 5'- cGCGCAGCAGauacgugaggGCgccgCgCCggGACUCGg -3' miRNA: 3'- -CGCGUCGUCaa--------CGa---G-GGuaCUGAGCg -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 10342 | 0.67 | 0.646022 |
Target: 5'- -gGCAGCcGUUGCgcagCCCGcacaUGAUcCGCa -3' miRNA: 3'- cgCGUCGuCAACGa---GGGU----ACUGaGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 4910 | 0.68 | 0.634871 |
Target: 5'- gGUGCGGUAGcUGCacugCCCGgcgcGGCgUCGCg -3' miRNA: 3'- -CGCGUCGUCaACGa---GGGUa---CUG-AGCG- -5' |
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12781 | 3' | -54.5 | NC_003387.1 | + | 2909 | 0.68 | 0.608119 |
Target: 5'- gGCGCGGCAGgccgcgggcgagGCUCUCGUcGaACUgCGCc -3' miRNA: 3'- -CGCGUCGUCaa----------CGAGGGUA-C-UGA-GCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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