Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12788 | 3' | -57 | NC_003387.1 | + | 5953 | 0.67 | 0.527656 |
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Target: 5'- cGCGAGGaCCGa--CCUCGGCGGGagaaAGg -3' miRNA: 3'- -UGCUCUaGGCaacGGAGCCGCUCg---UC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 3367 | 0.68 | 0.506777 |
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Target: 5'- gACGGcAUCaccaaGcugGCCUCGGCGGGCAu -3' miRNA: 3'- -UGCUcUAGg----Caa-CGGAGCCGCUCGUc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 24273 | 0.68 | 0.476146 |
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Target: 5'- cGCGAucugCCGccGCCUCGGCGuGCGc -3' miRNA: 3'- -UGCUcua-GGCaaCGGAGCCGCuCGUc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 31158 | 0.68 | 0.456244 |
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Target: 5'- uGCaGGAUCagcucgGUUGCCUCGGUgccGGGCGGc -3' miRNA: 3'- -UGcUCUAGg-----CAACGGAGCCG---CUCGUC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 18197 | 0.69 | 0.446463 |
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Target: 5'- uGCGAGGUgC---GCCUCGGCGAcGCGa -3' miRNA: 3'- -UGCUCUAgGcaaCGGAGCCGCU-CGUc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 49808 | 0.69 | 0.40855 |
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Target: 5'- gACGAGAgccucgcCCGcgGCCUgccgcgccCGGCGAGCGa -3' miRNA: 3'- -UGCUCUa------GGCaaCGGA--------GCCGCUCGUc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 39249 | 0.72 | 0.271654 |
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Target: 5'- gGCGAGG-CCGUcgaguucGaCCUCGGCGAGCuGg -3' miRNA: 3'- -UGCUCUaGGCAa------C-GGAGCCGCUCGuC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 21687 | 0.66 | 0.602825 |
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Target: 5'- cCGAcGccCUGgccGCCUCGGCGAGCGc -3' miRNA: 3'- uGCU-CuaGGCaa-CGGAGCCGCUCGUc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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