Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12788 | 3' | -57 | NC_003387.1 | + | 43784 | 0.7 | 0.39939 |
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Target: 5'- uGCGGGcgCCGUgcaucgcggugGCCUCGGacguGAGCAc -3' miRNA: 3'- -UGCUCuaGGCAa----------CGGAGCCg---CUCGUc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 19245 | 0.7 | 0.390363 |
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Target: 5'- cGCGGGcgCCGaccGCCUCGGCGAcuuCGGc -3' miRNA: 3'- -UGCUCuaGGCaa-CGGAGCCGCUc--GUC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 7956 | 0.7 | 0.372713 |
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Target: 5'- uCGAGGUCgGgccGCUUcaCGGCGGGCGGg -3' miRNA: 3'- uGCUCUAGgCaa-CGGA--GCCGCUCGUC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 17077 | 0.71 | 0.320752 |
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Target: 5'- uCGAGGUcggccagugcccgcCCGgccGCCUCGGCGGGCu- -3' miRNA: 3'- uGCUCUA--------------GGCaa-CGGAGCCGCUCGuc -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 39249 | 0.72 | 0.271654 |
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Target: 5'- gGCGAGG-CCGUcgaguucGaCCUCGGCGAGCuGg -3' miRNA: 3'- -UGCUCUaGGCAa------C-GGAGCCGCUCGuC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 48142 | 0.74 | 0.227002 |
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Target: 5'- uCGAGcu-UGUcgGCCUCGGCGAGCAGc -3' miRNA: 3'- uGCUCuagGCAa-CGGAGCCGCUCGUC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 51470 | 0.75 | 0.193823 |
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Target: 5'- cCGGGGUCCGUcgGCUUCGGUGgcguaccggccAGCAGg -3' miRNA: 3'- uGCUCUAGGCAa-CGGAGCCGC-----------UCGUC- -5' |
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| 12788 | 3' | -57 | NC_003387.1 | + | 45606 | 1.07 | 0.000834 |
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Target: 5'- gACGAGAUCCGUUGCCUCGGCGAGCAGc -3' miRNA: 3'- -UGCUCUAGGCAACGGAGCCGCUCGUC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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