Results 1 - 20 of 56 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12789 | 3' | -59 | NC_003387.1 | + | 44622 | 0.66 | 0.52573 |
Target: 5'- cCGUgGCgcgagcguguGGCCCUggccGCgGCCUCGGCGAu- -3' miRNA: 3'- -GCAgUG----------CCGGGG----UG-CGGAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 34130 | 0.66 | 0.515415 |
Target: 5'- aCGUCGCGGU---GCGCgaaCUCGACGAGc -3' miRNA: 3'- -GCAGUGCCGgggUGCG---GAGCUGCUUu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 22457 | 0.66 | 0.515415 |
Target: 5'- gCGU--UGGCCUCGCGguaCUCGACGAc- -3' miRNA: 3'- -GCAguGCCGGGGUGCg--GAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 48273 | 0.66 | 0.515415 |
Target: 5'- gGUCGgcuCGGCCUUcgGCGCgggCUCGGCGAAc -3' miRNA: 3'- gCAGU---GCCGGGG--UGCG---GAGCUGCUUu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 48299 | 0.66 | 0.505183 |
Target: 5'- aGUUc--GCCgCC-CGCCUCGACGAAAu -3' miRNA: 3'- gCAGugcCGG-GGuGCGGAGCUGCUUU- -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 2832 | 0.66 | 0.505183 |
Target: 5'- uCGUCaACGGCCgCgACGCCgaCGACa--- -3' miRNA: 3'- -GCAG-UGCCGG-GgUGCGGa-GCUGcuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 27790 | 0.66 | 0.505183 |
Target: 5'- ---gGCGGCCCCGcCGUCggucgCGGCGGu- -3' miRNA: 3'- gcagUGCCGGGGU-GCGGa----GCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 11340 | 0.66 | 0.495039 |
Target: 5'- gGUCGCccggcgcucGGCCUCACGC-UCGGCGu-- -3' miRNA: 3'- gCAGUG---------CCGGGGUGCGgAGCUGCuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 10942 | 0.66 | 0.495039 |
Target: 5'- gGUCGCGGCa-CGCGCC-CGGCu--- -3' miRNA: 3'- gCAGUGCCGggGUGCGGaGCUGcuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 22487 | 0.66 | 0.495039 |
Target: 5'- gCGUCguaccgggcgACGGCCUCggcgGCGCgCUCGACGu-- -3' miRNA: 3'- -GCAG----------UGCCGGGG----UGCG-GAGCUGCuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 11481 | 0.66 | 0.495039 |
Target: 5'- -uUCAUGGCCgacaAUGCCUCGugGGc- -3' miRNA: 3'- gcAGUGCCGGgg--UGCGGAGCugCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 41568 | 0.66 | 0.495039 |
Target: 5'- uCGUCACGuuGCCCauagGCGCCUUGAUc--- -3' miRNA: 3'- -GCAGUGC--CGGGg---UGCGGAGCUGcuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 22619 | 0.66 | 0.495039 |
Target: 5'- gGUCACuuGGCggCCCugGCCgcccgcCGACGAc- -3' miRNA: 3'- gCAGUG--CCG--GGGugCGGa-----GCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 30974 | 0.66 | 0.484988 |
Target: 5'- gGUgGCGcugacGCCgaGCGCCUCGGCGAc- -3' miRNA: 3'- gCAgUGC-----CGGggUGCGGAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 51054 | 0.67 | 0.465188 |
Target: 5'- uGUCGguacCGGCCCaCACcuCCUCGACGu-- -3' miRNA: 3'- gCAGU----GCCGGG-GUGc-GGAGCUGCuuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 43305 | 0.67 | 0.464209 |
Target: 5'- gCGUCGuacucguCGG-CCCACuCCUCGGCGAu- -3' miRNA: 3'- -GCAGU-------GCCgGGGUGcGGAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 37842 | 0.67 | 0.456416 |
Target: 5'- gGUCG-GGCaCCCcugcgcccggcggcuCGCCUCGACGAu- -3' miRNA: 3'- gCAGUgCCG-GGGu--------------GCGGAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 18590 | 0.67 | 0.455446 |
Target: 5'- gCGUCAgGGUCgCC-CGCCUCGGUGGc- -3' miRNA: 3'- -GCAGUgCCGG-GGuGCGGAGCUGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 34837 | 0.67 | 0.455446 |
Target: 5'- uCGUCgACGGCCCgCGCggGCCUUG-CGGu- -3' miRNA: 3'- -GCAG-UGCCGGG-GUG--CGGAGCuGCUuu -5' |
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12789 | 3' | -59 | NC_003387.1 | + | 37416 | 0.67 | 0.454478 |
Target: 5'- aCGUCGCGauggcgaccaagaGCCCCAaGCCgcCGACGGu- -3' miRNA: 3'- -GCAGUGC-------------CGGGGUgCGGa-GCUGCUuu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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