Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 12799 | 5' | -54.4 | NC_003387.1 | + | 40109 | 0.66 | 0.74976 |
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Target: 5'- cUCGcagGCGGCGg--CC-CGGCGUCGGg -3' miRNA: 3'- cAGCa--CGUUGUagaGGaGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 19119 | 0.66 | 0.74976 |
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Target: 5'- gGUCGUGCcACA---CCUCGGCGg-GAu -3' miRNA: 3'- -CAGCACGuUGUagaGGAGCCGCagCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 20427 | 0.66 | 0.74976 |
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Target: 5'- gGUUGUGCAGCcgccggugCUCCUCGcGCccccacgccuuGUCGGc -3' miRNA: 3'- -CAGCACGUUGua------GAGGAGC-CG-----------CAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 43315 | 0.66 | 0.74976 |
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Target: 5'- cGUCG-GCc-CA-CUCCUCGGCGaUCGu -3' miRNA: 3'- -CAGCaCGuuGUaGAGGAGCCGC-AGCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 21030 | 0.66 | 0.74976 |
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Target: 5'- gGUCGuUGCuACGcCUgaCCagUCGGCGUCGAa -3' miRNA: 3'- -CAGC-ACGuUGUaGA--GG--AGCCGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 11618 | 0.66 | 0.753927 |
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Target: 5'- gGUCGUGCAGCGccagcagcucgcggcUCacgaucgccCCUCGaucGCGUCGAc -3' miRNA: 3'- -CAGCACGUUGU---------------AGa--------GGAGC---CGCAGCU- -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 50303 | 0.66 | 0.770388 |
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Target: 5'- -gCGcUGCcACAUCUCCcaggCGGgGUCGu -3' miRNA: 3'- caGC-ACGuUGUAGAGGa---GCCgCAGCu -5' |
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| 12799 | 5' | -54.4 | NC_003387.1 | + | 8918 | 0.66 | 0.770388 |
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Target: 5'- aUCGUGCAGCA-CUCgUCGaggaaGCGggCGAc -3' miRNA: 3'- cAGCACGUUGUaGAGgAGC-----CGCa-GCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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