Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 12872 | 5' | -47.7 | NC_003387.1 | + | 43526 | 0.79 | 0.430112 |
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Target: 5'- gGGGUGccggucugUGAGGUGUCAGC-UAGACGg -3' miRNA: 3'- -CCCACa-------ACUCCACAGUUGuGUUUGCg -5' |
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| 12872 | 5' | -47.7 | NC_003387.1 | + | 45122 | 0.66 | 0.986736 |
|
Target: 5'- cGGUGUacGAGGUGacgcauuUCGAgGCGcGGCGCu -3' miRNA: 3'- cCCACAa-CUCCAC-------AGUUgUGU-UUGCG- -5' |
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| 12872 | 5' | -47.7 | NC_003387.1 | + | 46936 | 0.77 | 0.525801 |
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Target: 5'- cGGGcaccGAGGUGUCGAUccgcgGCGAGCGCg -3' miRNA: 3'- -CCCacaaCUCCACAGUUG-----UGUUUGCG- -5' |
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| 12872 | 5' | -47.7 | NC_003387.1 | + | 47204 | 0.66 | 0.985036 |
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Target: 5'- aGGUGgccgccGGUGUCGGCAUuGugGUg -3' miRNA: 3'- cCCACaacu--CCACAGUUGUGuUugCG- -5' |
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| 12872 | 5' | -47.7 | NC_003387.1 | + | 47338 | 0.67 | 0.972432 |
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Target: 5'- -cGUGUUGAcagGUCAACACc-ACGCa -3' miRNA: 3'- ccCACAACUccaCAGUUGUGuuUGCG- -5' |
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| 12872 | 5' | -47.7 | NC_003387.1 | + | 50006 | 0.69 | 0.927392 |
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Target: 5'- -aGUGUUGAGGUaUCAACAgCAcGACGg -3' miRNA: 3'- ccCACAACUCCAcAGUUGU-GU-UUGCg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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