Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
12989 | 5' | -57.6 | NC_003387.1 | + | 32228 | 0.66 | 0.553764 |
Target: 5'- -gCUGCgcgagcgcgaCGAGCUGGCCGAcCGcCUGGc -3' miRNA: 3'- uaGACGg---------GCUCGACUGGCU-GCuGACU- -5' |
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12989 | 5' | -57.6 | NC_003387.1 | + | 22514 | 0.66 | 0.553764 |
Target: 5'- gAUCaUGCCCGccgcgacccuGGCccaGGCCGAgGGCUGGc -3' miRNA: 3'- -UAG-ACGGGC----------UCGa--CUGGCUgCUGACU- -5' |
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12989 | 5' | -57.6 | NC_003387.1 | + | 23878 | 0.66 | 0.553764 |
Target: 5'- ---cGaCCCGguguGGCUGGCCGACGuGCUGc -3' miRNA: 3'- uagaC-GGGC----UCGACUGGCUGC-UGACu -5' |
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12989 | 5' | -57.6 | NC_003387.1 | + | 32609 | 0.66 | 0.564511 |
Target: 5'- -cCUGCUCGGGCUcGCCG-CGggggcGCUGAc -3' miRNA: 3'- uaGACGGGCUCGAcUGGCuGC-----UGACU- -5' |
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12989 | 5' | -57.6 | NC_003387.1 | + | 31720 | 0.66 | 0.575311 |
Target: 5'- ---aGCCCGGcGCUGucGCCGACGAgaGc -3' miRNA: 3'- uagaCGGGCU-CGAC--UGGCUGCUgaCu -5' |
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12989 | 5' | -57.6 | NC_003387.1 | + | 32162 | 0.65 | 0.581814 |
Target: 5'- -cCUGCaggcacagauugaCGAGCUGACCGcCGAgCUGc -3' miRNA: 3'- uaGACGg------------GCUCGACUGGCuGCU-GACu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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