Results 1 - 20 of 40 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13099 | 5' | -62.7 | NC_003401.1 | + | 132302 | 0.66 | 0.625834 |
Target: 5'- cGCGCCgggCGCCGccGGCcu--CCCCCg -3' miRNA: 3'- cUGCGGa--GCGGCauCCGcugcGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 91631 | 0.66 | 0.616042 |
Target: 5'- aGACGCCUCGC-----GCGACGUucaaCCCg -3' miRNA: 3'- -CUGCGGAGCGgcaucCGCUGCGg---GGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 16309 | 0.66 | 0.606262 |
Target: 5'- cGCGUCUCgGUCGcgAGGUGAcCGCCCg- -3' miRNA: 3'- cUGCGGAG-CGGCa-UCCGCU-GCGGGgg -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 103725 | 0.66 | 0.606262 |
Target: 5'- gGACGCaaucaaCUCGCCGgccaacgucGUGACuCCCCCg -3' miRNA: 3'- -CUGCG------GAGCGGCauc------CGCUGcGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 51739 | 0.66 | 0.603331 |
Target: 5'- cGAgGCCUCGaaCGgcGGgGACGguucaguuucaagaCCCCCa -3' miRNA: 3'- -CUgCGGAGCg-GCauCCgCUGC--------------GGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 32256 | 0.66 | 0.600401 |
Target: 5'- cACGUCUccguccggaaCGCCGUGGGaauuacugcacccgGACGCCCUg -3' miRNA: 3'- cUGCGGA----------GCGGCAUCCg-------------CUGCGGGGg -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 87150 | 0.66 | 0.58676 |
Target: 5'- --gGCCUUGCuCGgcGGUGucaGCUCCCu -3' miRNA: 3'- cugCGGAGCG-GCauCCGCug-CGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 128407 | 0.66 | 0.567378 |
Target: 5'- aGAC-CCaauaugCGCCGc-GGUGAUGCUCCCg -3' miRNA: 3'- -CUGcGGa-----GCGGCauCCGCUGCGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 46775 | 0.66 | 0.567378 |
Target: 5'- -uCGCCUCGUCGauauauaggAGGUGGaacgucUGUCCCCg -3' miRNA: 3'- cuGCGGAGCGGCa--------UCCGCU------GCGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 119778 | 0.67 | 0.557747 |
Target: 5'- cGACgGCCUCcgccaaucugGCCGgAGGCGGCGCagcggcaCCg -3' miRNA: 3'- -CUG-CGGAG----------CGGCaUCCGCUGCGgg-----GG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 127829 | 0.67 | 0.548163 |
Target: 5'- -uCGCCUCGCUGguGGUGGCGCUgUg -3' miRNA: 3'- cuGCGGAGCGGCauCCGCUGCGGgGg -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 23800 | 0.67 | 0.548163 |
Target: 5'- cGGCGCCaccUGCCGgccgcucGCGGCGCCCg- -3' miRNA: 3'- -CUGCGGa--GCGGCauc----CGCUGCGGGgg -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 105671 | 0.67 | 0.548163 |
Target: 5'- cACGCCgucuaUCGUCGUgagcgcccAGGUGACGgCCgCCa -3' miRNA: 3'- cUGCGG-----AGCGGCA--------UCCGCUGCgGG-GG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 87879 | 0.67 | 0.53768 |
Target: 5'- cACGgCUCGCCuuccggaGUGGGa-ACGCCUCCu -3' miRNA: 3'- cUGCgGAGCGG-------CAUCCgcUGCGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 89672 | 0.67 | 0.529156 |
Target: 5'- --aGCCUcCGCCGgagcgggaggAGGCaggGGCGUCCCUg -3' miRNA: 3'- cugCGGA-GCGGCa---------UCCG---CUGCGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 89868 | 0.67 | 0.529156 |
Target: 5'- aGGCgGCCUgCGuCCaUGGGCuugagccaggGACGCCCCUg -3' miRNA: 3'- -CUG-CGGA-GC-GGcAUCCG----------CUGCGGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 13001 | 0.67 | 0.519744 |
Target: 5'- --gGCCaaGuUCGUGGGCGACGCCaucuCCg -3' miRNA: 3'- cugCGGagC-GGCAUCCGCUGCGGg---GG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 21231 | 0.68 | 0.501122 |
Target: 5'- uGAC-CCUCGuaGUuGGCGuCGgCCCCCc -3' miRNA: 3'- -CUGcGGAGCggCAuCCGCuGC-GGGGG- -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 132681 | 0.68 | 0.497433 |
Target: 5'- gGGCGCCUCGgCGgggcccggcgcggGGcGCGACcgaggGCCCCg -3' miRNA: 3'- -CUGCGGAGCgGCa------------UC-CGCUG-----CGGGGg -5' |
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13099 | 5' | -62.7 | NC_003401.1 | + | 118655 | 0.68 | 0.482801 |
Target: 5'- uGAgGUgUCGCCgGUGGGcCGuAUGaCCCCCg -3' miRNA: 3'- -CUgCGgAGCGG-CAUCC-GC-UGC-GGGGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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