Results 1 - 20 of 33 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13109 | 3' | -61.7 | NC_003401.1 | + | 49871 | 0.66 | 0.619818 |
Target: 5'- uGuCGGGCGUcacGGCCGaaggGaCCGAGGGCa- -3' miRNA: 3'- gC-GUCUGCG---CCGGCa---C-GGUUCCCGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 109918 | 0.66 | 0.609831 |
Target: 5'- -aUAGACGCaGCCGUGCUGcAGGcGCa- -3' miRNA: 3'- gcGUCUGCGcCGGCACGGU-UCC-CGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 126954 | 0.66 | 0.609831 |
Target: 5'- gGUAaucuGACaGCGGCCG-GCCcAGGGgUCc -3' miRNA: 3'- gCGU----CUG-CGCCGGCaCGGuUCCCgAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 105000 | 0.66 | 0.603845 |
Target: 5'- aCGCcucuaGGACGUuugaguacgugaucuGGCgGUGCagAGGGGCUCc -3' miRNA: 3'- -GCG-----UCUGCG---------------CCGgCACGg-UUCCCGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 50027 | 0.66 | 0.599859 |
Target: 5'- aGCGaACuCGGCCGccGUCcGGGGCUCg -3' miRNA: 3'- gCGUcUGcGCCGGCa-CGGuUCCCGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 132398 | 0.66 | 0.589911 |
Target: 5'- gCGCaAGAUgGCGGCCGcgGCCcGGcGGCg- -3' miRNA: 3'- -GCG-UCUG-CGCCGGCa-CGGuUC-CCGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 132238 | 0.66 | 0.579991 |
Target: 5'- cCGcCGGGcCGCGGCCGccaucuugcGCCcGGGGCg- -3' miRNA: 3'- -GC-GUCU-GCGCCGGCa--------CGGuUCCCGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 14793 | 0.66 | 0.579991 |
Target: 5'- aCGCGGcACGgcCGGCUGUGUUcuuGAGGGCc- -3' miRNA: 3'- -GCGUC-UGC--GCCGGCACGG---UUCCCGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 42295 | 0.67 | 0.550472 |
Target: 5'- aGCGGguggaGCGUGGCCGccaCCAGGGGaUCg -3' miRNA: 3'- gCGUC-----UGCGCCGGCac-GGUUCCCgAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 32425 | 0.67 | 0.521429 |
Target: 5'- --gAGACGCGGCa--GCUuaccGGGGCUCa -3' miRNA: 3'- gcgUCUGCGCCGgcaCGGu---UCCCGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 89799 | 0.67 | 0.521429 |
Target: 5'- uGCGG-CGCGGuCCGUauauGCCA-GGGC-Cg -3' miRNA: 3'- gCGUCuGCGCC-GGCA----CGGUuCCCGaG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 132648 | 0.68 | 0.502402 |
Target: 5'- cCGcCGGGCuCGGCCG-GaCCGGGGcGCUCc -3' miRNA: 3'- -GC-GUCUGcGCCGGCaC-GGUUCC-CGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 72160 | 0.68 | 0.502402 |
Target: 5'- gGUAGACGCGcaCCGUGagaCGAGGGUc- -3' miRNA: 3'- gCGUCUGCGCc-GGCACg--GUUCCCGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 24751 | 0.68 | 0.493002 |
Target: 5'- cCGCAG-CGCgGGgCGUGUUAAgcGGGCUUg -3' miRNA: 3'- -GCGUCuGCG-CCgGCACGGUU--CCCGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 132498 | 0.68 | 0.493002 |
Target: 5'- cCGCGGGCcCgGGCCGgcgggGCgGAGGGC-Cg -3' miRNA: 3'- -GCGUCUGcG-CCGGCa----CGgUUCCCGaG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 132556 | 0.68 | 0.465307 |
Target: 5'- cCGCGGcGCGCGGCuccCGaUGCCGGGcGGC-Cg -3' miRNA: 3'- -GCGUC-UGCGCCG---GC-ACGGUUC-CCGaG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 14625 | 0.68 | 0.464398 |
Target: 5'- aGCAGGCccucaagaacacaGcCGGCCGUGCCGcguGcGGCUUc -3' miRNA: 3'- gCGUCUG-------------C-GCCGGCACGGUu--C-CCGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 122426 | 0.69 | 0.456256 |
Target: 5'- gCGCAGcGCGCGGCCGcUGCCGAaaccaacgccGCUUg -3' miRNA: 3'- -GCGUC-UGCGCCGGC-ACGGUUcc--------CGAG- -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 118439 | 0.69 | 0.447301 |
Target: 5'- gGCAGAgGcCGGCCG-GCCAaAGGuGCg- -3' miRNA: 3'- gCGUCUgC-GCCGGCaCGGU-UCC-CGag -5' |
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13109 | 3' | -61.7 | NC_003401.1 | + | 124501 | 0.69 | 0.438444 |
Target: 5'- gGguGACGCagGGCCGU-CCuauAGaGGCUCg -3' miRNA: 3'- gCguCUGCG--CCGGCAcGGu--UC-CCGAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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