Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 13281 | 3' | -52.3 | NC_003409.1 | + | 8943 | 0.69 | 0.890778 |
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Target: 5'- gGGUGuGGGgGAAcuggacgucugaaagGUGUCACCccucGAGUGCg -3' miRNA: 3'- -CCAC-CCCgUUUa--------------CACAGUGG----UUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 46175 | 0.69 | 0.896928 |
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Target: 5'- uGGgccGGGGCGaugacuGAUGaGUaGCCAAGUGCg -3' miRNA: 3'- -CCa--CCCCGU------UUACaCAgUGGUUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 110033 | 0.69 | 0.903528 |
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Target: 5'- --aGGGGUAcg-GUGgcaguguugCGCCGGGCGCc -3' miRNA: 3'- ccaCCCCGUuuaCACa--------GUGGUUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 71558 | 0.69 | 0.909879 |
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Target: 5'- gGGcGGGGCuuAAUGaGUCGCCGGuaGCu -3' miRNA: 3'- -CCaCCCCGu-UUACaCAGUGGUUcgCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 22421 | 0.68 | 0.94267 |
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Target: 5'- --cGGGGCGGAaaguaUGcggGUCGCCugcgaagcGGCGCa -3' miRNA: 3'- ccaCCCCGUUU-----ACa--CAGUGGu-------UCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 111322 | 0.66 | 0.980168 |
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Target: 5'- cGGgGGGGCu--UGUGUC-UCucGCGUg -3' miRNA: 3'- -CCaCCCCGuuuACACAGuGGuuCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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