Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13281 | 3' | -52.3 | NC_003409.1 | + | 115682 | 0.66 | 0.977598 |
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Target: 5'- uGUGGGGCGAcuugcgccgcagaAUGUGgC-CCGGGCu- -3' miRNA: 3'- cCACCCCGUU-------------UACACaGuGGUUCGcg -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 116842 | 0.66 | 0.977598 |
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Target: 5'- cGGUGGGauuuucuGUGGAUGgg-CACaGAGCGCa -3' miRNA: 3'- -CCACCC-------CGUUUACacaGUGgUUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 36132 | 0.66 | 0.977841 |
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Target: 5'- gGGUGuGGGCAAAUcaacgcugGUCaacGCCGuGUGCg -3' miRNA: 3'- -CCAC-CCCGUUUAca------CAG---UGGUuCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 59908 | 0.66 | 0.977841 |
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Target: 5'- gGGUcGGGGUGAG-GUGcuUCGCUGAguuGCGCa -3' miRNA: 3'- -CCA-CCCCGUUUaCAC--AGUGGUU---CGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 133477 | 0.66 | 0.979944 |
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Target: 5'- cGGUGaGGGCccccUGUGUCugUccgccuggaaacaGGGUGCu -3' miRNA: 3'- -CCAC-CCCGuuu-ACACAGugG-------------UUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 111322 | 0.66 | 0.980168 |
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Target: 5'- cGGgGGGGCu--UGUGUC-UCucGCGUg -3' miRNA: 3'- -CCaCCCCGuuuACACAGuGGuuCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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