Results 41 - 46 of 46 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13281 | 3' | -52.3 | NC_003409.1 | + | 10085 | 0.72 | 0.800147 |
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Target: 5'- cGUGGGGUucccgcUGUCGCCcuGAGCGUg -3' miRNA: 3'- cCACCCCGuuuac-ACAGUGG--UUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 90952 | 0.72 | 0.800147 |
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Target: 5'- gGGUGGGGCAAAUGguuugGcCACCcuAGUcCg -3' miRNA: 3'- -CCACCCCGUUUACa----CaGUGGu-UCGcG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 71596 | 0.73 | 0.721905 |
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Target: 5'- aGGUGGGaGCAGA-GU-UCGCCAAGCu- -3' miRNA: 3'- -CCACCC-CGUUUaCAcAGUGGUUCGcg -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 82254 | 0.74 | 0.690932 |
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Target: 5'- gGGUGGGG-GGAUG-GUcCGCCGGGCGa -3' miRNA: 3'- -CCACCCCgUUUACaCA-GUGGUUCGCg -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 75189 | 0.81 | 0.333658 |
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Target: 5'- aGUGagcauGGCAGAUGUucGUCACCGGGCGCg -3' miRNA: 3'- cCACc----CCGUUUACA--CAGUGGUUCGCG- -5' |
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| 13281 | 3' | -52.3 | NC_003409.1 | + | 30105 | 1.14 | 0.002746 |
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Target: 5'- cGGUGGGGCAAAUGUGUCACCAAGCGCc -3' miRNA: 3'- -CCACCCCGUUUACACAGUGGUUCGCG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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