Results 21 - 24 of 24 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13282 | 5' | -54.3 | NC_003409.1 | + | 14197 | 0.72 | 0.600771 |
Target: 5'- gCUCCgAGCugGCAGAGCAccCCGAg-- -3' miRNA: 3'- gGAGG-UCGugCGUCUCGUa-GGUUguu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 11863 | 0.66 | 0.924722 |
Target: 5'- cCCUCCAgGCugGCuucGGucgcGCAUCCuGCGg -3' miRNA: 3'- -GGAGGU-CGugCG---UCu---CGUAGGuUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 11012 | 0.71 | 0.695648 |
Target: 5'- aCCUCCuAGCG-GCGGAGCc-CCAACAc -3' miRNA: 3'- -GGAGG-UCGUgCGUCUCGuaGGUUGUu -5' |
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13282 | 5' | -54.3 | NC_003409.1 | + | 4164 | 0.7 | 0.730667 |
Target: 5'- gCCUUCgugcacuagaaguuuGGCACGCAGAGCAggCCuuGCAu -3' miRNA: 3'- -GGAGG---------------UCGUGCGUCUCGUa-GGu-UGUu -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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