Results 1 - 20 of 41 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13284 | 5' | -56.6 | NC_003409.1 | + | 38617 | 0.66 | 0.838853 |
Target: 5'- uUGCGcGCcgaGGC-GCCGCAGUc-GUCCGCu -3' miRNA: 3'- -ACGU-CG---UCGuCGGCGUCAucUAGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 32010 | 0.66 | 0.838013 |
Target: 5'- aGCAcGUaAGUAGCUGCAGgcUGGAggaccuuUCCACa -3' miRNA: 3'- aCGU-CG-UCGUCGGCGUC--AUCU-------AGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 43788 | 0.66 | 0.830361 |
Target: 5'- cGUAGCuGGCguaGGCCGCGGgcccGGAcauggcacuuUCCACu -3' miRNA: 3'- aCGUCG-UCG---UCGGCGUCa---UCU----------AGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 59962 | 0.66 | 0.830361 |
Target: 5'- cGCGGCGGUGGCCGUAuaucGggCCAa -3' miRNA: 3'- aCGUCGUCGUCGGCGUcau-CuaGGUg -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 14041 | 0.66 | 0.821678 |
Target: 5'- cGCGGCcuugacgcucGGCucCCGCAGUaugAGGUCCAg -3' miRNA: 3'- aCGUCG----------UCGucGGCGUCA---UCUAGGUg -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 49221 | 0.66 | 0.821678 |
Target: 5'- aUGCAGCAGUAGaCGCAGgcGc-CUAUg -3' miRNA: 3'- -ACGUCGUCGUCgGCGUCauCuaGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 113521 | 0.66 | 0.821678 |
Target: 5'- gUGCGgcGCAGCAGCUGCu-UGGcgcCCACg -3' miRNA: 3'- -ACGU--CGUCGUCGGCGucAUCua-GGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 125091 | 0.66 | 0.818154 |
Target: 5'- aGCAGCAGCAGCaggaugagcagcaGCAGcaGGAUgaACa -3' miRNA: 3'- aCGUCGUCGUCGg------------CGUCa-UCUAggUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 33013 | 0.66 | 0.812814 |
Target: 5'- cGCaAGgAGCAGCCuGCAGgGGAgcaCACu -3' miRNA: 3'- aCG-UCgUCGUCGG-CGUCaUCUag-GUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 31197 | 0.66 | 0.812814 |
Target: 5'- cUGCGGCuGCuGCUGCAGaGGAggaCgGCg -3' miRNA: 3'- -ACGUCGuCGuCGGCGUCaUCUa--GgUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 125145 | 0.66 | 0.790854 |
Target: 5'- aGCAGCAGCAGCaggaugagcagcaGCAGcaGGAUgaGCa -3' miRNA: 3'- aCGUCGUCGUCGg------------CGUCa-UCUAggUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 85872 | 0.67 | 0.785223 |
Target: 5'- gGUGGCGGCAGUgcgaCGCA--AGuAUCCACa -3' miRNA: 3'- aCGUCGUCGUCG----GCGUcaUC-UAGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 69528 | 0.67 | 0.746455 |
Target: 5'- gGCuaaaAGCAGCAGCCGgAG-AGcgCaCGCa -3' miRNA: 3'- aCG----UCGUCGUCGGCgUCaUCuaG-GUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 106382 | 0.68 | 0.740479 |
Target: 5'- aGCAGUGGCAG-CGCucuGUAGGuaugccuguagacuuUCCACc -3' miRNA: 3'- aCGUCGUCGUCgGCGu--CAUCU---------------AGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 19218 | 0.68 | 0.736475 |
Target: 5'- cGUAGUAcGCGGCUGCAGcgacguGAUCUAUc -3' miRNA: 3'- aCGUCGU-CGUCGGCGUCau----CUAGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 125981 | 0.68 | 0.716237 |
Target: 5'- aGCAGCAGgAGCCacaacaGCAGgag--CCACa -3' miRNA: 3'- aCGUCGUCgUCGG------CGUCaucuaGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 93544 | 0.68 | 0.695697 |
Target: 5'- gGCGGCGGCAGUCGCcacgc--CCACa -3' miRNA: 3'- aCGUCGUCGUCGGCGucaucuaGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 109944 | 0.69 | 0.674934 |
Target: 5'- cGCAGCAGUccagaAGCCGUGucGGAUUCAUc -3' miRNA: 3'- aCGUCGUCG-----UCGGCGUcaUCUAGGUG- -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 132814 | 0.69 | 0.674934 |
Target: 5'- gGCGcGCGGCGcGCUGCAGUAccUCCAa -3' miRNA: 3'- aCGU-CGUCGU-CGGCGUCAUcuAGGUg -5' |
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13284 | 5' | -56.6 | NC_003409.1 | + | 47330 | 0.69 | 0.654023 |
Target: 5'- cUGCAGCAGCuGuuGguGUA---CCACa -3' miRNA: 3'- -ACGUCGUCGuCggCguCAUcuaGGUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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