Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13293 | 3' | -56.2 | NC_003409.1 | + | 133546 | 0.7 | 0.645633 |
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Target: 5'- aGGGCGUacaGCAGGC-CGAGAUGCu- -3' miRNA: 3'- aCCCGUAcg-UGUCUGcGCUCUACGcc -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 16336 | 0.71 | 0.635345 |
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Target: 5'- uUGGGCAUGCAcCAGG-GUGAGA-GCa- -3' miRNA: 3'- -ACCCGUACGU-GUCUgCGCUCUaCGcc -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 118467 | 0.72 | 0.573837 |
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Target: 5'- cGGGUuc-CugGGGUGCGGGGUGCGGg -3' miRNA: 3'- aCCCGuacGugUCUGCGCUCUACGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 23959 | 0.72 | 0.56369 |
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Target: 5'- cGGGguUGUuCGGugGCGGGggGgGGg -3' miRNA: 3'- aCCCguACGuGUCugCGCUCuaCgCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 88022 | 0.72 | 0.533567 |
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Target: 5'- -cGGCGUcGCACAGACGCcggGGGAUaCGGg -3' miRNA: 3'- acCCGUA-CGUGUCUGCG---CUCUAcGCC- -5' |
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| 13293 | 3' | -56.2 | NC_003409.1 | + | 38284 | 1.1 | 0.002202 |
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Target: 5'- gUGGGCAUGCACAGACGCGAGAUGCGGc -3' miRNA: 3'- -ACCCGUACGUGUCUGCGCUCUACGCC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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