Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1332 | 3' | -61.9 | NC_001335.1 | + | 32921 | 0.67 | 0.307985 |
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Target: 5'- gCGguGCUCCACgGUCGUCG-UAGGACa -3' miRNA: 3'- -GUguCGGGGUG-CGGCAGCgGUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 2792 | 0.67 | 0.307985 |
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Target: 5'- gACGGCCgCACGCaccaGcUCGUCAaGGACa -3' miRNA: 3'- gUGUCGGgGUGCGg---C-AGCGGU-CCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 688 | 0.68 | 0.293641 |
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Target: 5'- gCGCuGCaCUCACGCCGgauuucaGCCucuGGACGa -3' miRNA: 3'- -GUGuCG-GGGUGCGGCag-----CGGu--CCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 7063 | 0.68 | 0.265877 |
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Target: 5'- -uCAGCCgcguggaucaccgUCGCgGCCGUCaGCCAGGACc -3' miRNA: 3'- guGUCGG-------------GGUG-CGGCAG-CGGUCCUGc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 37900 | 0.68 | 0.260075 |
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Target: 5'- -cCGGCgUCGuCGCCGUCgGCCAGGAg- -3' miRNA: 3'- guGUCGgGGU-GCGGCAG-CGGUCCUgc -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 25908 | 0.74 | 0.099019 |
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Target: 5'- gACGGCCUgACGCuCGUCGCCAcGGuCGc -3' miRNA: 3'- gUGUCGGGgUGCG-GCAGCGGU-CCuGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 42043 | 0.77 | 0.063784 |
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Target: 5'- --gAGUCCCACGCCuUCGCCAGGGUGg -3' miRNA: 3'- gugUCGGGGUGCGGcAGCGGUCCUGC- -5' |
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| 1332 | 3' | -61.9 | NC_001335.1 | + | 22779 | 0.66 | 0.399785 |
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Target: 5'- gACAGCCaCCgacuaccagaagaugACGuuGgUCGUCGGGACu -3' miRNA: 3'- gUGUCGG-GG---------------UGCggC-AGCGGUCCUGc -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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