Results 21 - 26 of 26 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13327 | 5' | -64 | NC_003409.1 | + | 111137 | 0.69 | 0.360218 |
Target: 5'- -cGCcGGCCUucGCCcucaguccggggGGGCugGaGCGCCGu -3' miRNA: 3'- caCGuCCGGA--UGG------------CCCGugC-CGCGGC- -5' |
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13327 | 5' | -64 | NC_003409.1 | + | 100085 | 0.7 | 0.316481 |
Target: 5'- cGUGCGGGCCaaUGCCauGGCGgGGCgGCUGu -3' miRNA: 3'- -CACGUCCGG--AUGGc-CCGUgCCG-CGGC- -5' |
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13327 | 5' | -64 | NC_003409.1 | + | 101065 | 0.7 | 0.302819 |
Target: 5'- cUGCaugAGGCCUugC-GGCA-GGCGCCGu -3' miRNA: 3'- cACG---UCCGGAugGcCCGUgCCGCGGC- -5' |
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13327 | 5' | -64 | NC_003409.1 | + | 102714 | 0.73 | 0.204735 |
Target: 5'- -cGcCAGGUUUGCC-GGUACGGCGCCu -3' miRNA: 3'- caC-GUCCGGAUGGcCCGUGCCGCGGc -5' |
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13327 | 5' | -64 | NC_003409.1 | + | 5208 | 0.75 | 0.149461 |
Target: 5'- -aGUaaaGGGUCagaaaaACCGGGCACGGCGCCu -3' miRNA: 3'- caCG---UCCGGa-----UGGCCCGUGCCGCGGc -5' |
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13327 | 5' | -64 | NC_003409.1 | + | 20808 | 0.66 | 0.533233 |
Target: 5'- --cCAGGgaguuCCUgGCCGGGCGCGGC-CUGg -3' miRNA: 3'- cacGUCC-----GGA-UGGCCCGUGCCGcGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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