Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13327 | 5' | -64 | NC_003409.1 | + | 25931 | 0.66 | 0.486592 |
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Target: 5'- -cGC-GGUUgGCC-GGCGCGGCGUCGg -3' miRNA: 3'- caCGuCCGGaUGGcCCGUGCCGCGGC- -5' |
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| 13327 | 5' | -64 | NC_003409.1 | + | 40794 | 0.66 | 0.505032 |
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Target: 5'- --aCAGGCC--CCGGGCGgCGGCucGCCu -3' miRNA: 3'- cacGUCCGGauGGCCCGU-GCCG--CGGc -5' |
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| 13327 | 5' | -64 | NC_003409.1 | + | 104854 | 0.66 | 0.514364 |
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Target: 5'- -cGCgAGGUCUGCgCGGGCGCGuuuCGUCu -3' miRNA: 3'- caCG-UCCGGAUG-GCCCGUGCc--GCGGc -5' |
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| 13327 | 5' | -64 | NC_003409.1 | + | 41724 | 0.66 | 0.533233 |
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Target: 5'- uUGCAGGCgUgcuuccaacgauACCGGcCACGGaCGCgCGg -3' miRNA: 3'- cACGUCCGgA------------UGGCCcGUGCC-GCG-GC- -5' |
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| 13327 | 5' | -64 | NC_003409.1 | + | 31236 | 0.66 | 0.533233 |
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Target: 5'- cGUGCAGGCgCcACaCGGucaguuccaGCaaGCGGCGCCc -3' miRNA: 3'- -CACGUCCG-GaUG-GCC---------CG--UGCCGCGGc -5' |
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| 13327 | 5' | -64 | NC_003409.1 | + | 20808 | 0.66 | 0.533233 |
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Target: 5'- --cCAGGgaguuCCUgGCCGGGCGCGGC-CUGg -3' miRNA: 3'- cacGUCC-----GGA-UGGCCCGUGCCGcGGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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