Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13367 | 3' | -56.6 | NC_003409.1 | + | 114394 | 0.66 | 0.845572 |
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Target: 5'- ---uGGCGACAGCg-GGGUGgGGCu- -3' miRNA: 3'- ucauUUGCUGUCGagCCCGCgCCGcu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 87590 | 0.66 | 0.853509 |
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Target: 5'- -----cCGACAaauguucccGCUUGGGCguGCGGCGGa -3' miRNA: 3'- ucauuuGCUGU---------CGAGCCCG--CGCCGCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 20817 | 0.66 | 0.858945 |
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Target: 5'- --aAAAUGGCuccagggaguuccuGGC-CGGGCGCGGCc- -3' miRNA: 3'- ucaUUUGCUG--------------UCGaGCCCGCGCCGcu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 35917 | 0.66 | 0.861245 |
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Target: 5'- uGUAggUGACcGCUCaGGGa-CGGCGGc -3' miRNA: 3'- uCAUuuGCUGuCGAG-CCCgcGCCGCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 40606 | 0.66 | 0.861245 |
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Target: 5'- gAGUuccGGACGugAagGCUagucuaguagaGGGCGCGGCGc -3' miRNA: 3'- -UCA---UUUGCugU--CGAg----------CCCGCGCCGCu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 91880 | 0.66 | 0.87609 |
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Target: 5'- uAGUAGGCccauGugGGCUUGGGaGUGGCc- -3' miRNA: 3'- -UCAUUUG----CugUCGAGCCCgCGCCGcu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 87366 | 0.66 | 0.883187 |
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Target: 5'- uGUAAcAUGAUGGUUucgcauaccCGGGCGCGGgGGc -3' miRNA: 3'- uCAUU-UGCUGUCGA---------GCCCGCGCCgCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 115766 | 0.66 | 0.883187 |
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Target: 5'- ----uGCGAuCAGUcgaGGGCGCGGCu- -3' miRNA: 3'- ucauuUGCU-GUCGag-CCCGCGCCGcu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 88689 | 0.66 | 0.883187 |
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Target: 5'- -----cCGGCAgGCagGGGCGCGGgGGc -3' miRNA: 3'- ucauuuGCUGU-CGagCCCGCGCCgCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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