Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13367 | 3' | -56.6 | NC_003409.1 | + | 87169 | 0.69 | 0.717703 |
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Target: 5'- gGGUGGuGCGcCGGCggaGGGCGUGGCcGAc -3' miRNA: 3'- -UCAUU-UGCuGUCGag-CCCGCGCCG-CU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 41989 | 0.7 | 0.646804 |
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Target: 5'- uGUGAGCGAUGGCac-GGCGCaGCGAg -3' miRNA: 3'- uCAUUUGCUGUCGagcCCGCGcCGCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 35366 | 0.71 | 0.626313 |
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Target: 5'- --gGAGCGGacuCGGUg-GGGCGCGGCGGa -3' miRNA: 3'- ucaUUUGCU---GUCGagCCCGCGCCGCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 33645 | 0.71 | 0.60584 |
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Target: 5'- aGGUucAAGCGGCGGUUUGGGUGCccucgcGCGAc -3' miRNA: 3'- -UCA--UUUGCUGUCGAGCCCGCGc-----CGCU- -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 67791 | 0.72 | 0.545114 |
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Target: 5'- aGGUcGACGAUcGCggUGGGUGCGGCGc -3' miRNA: 3'- -UCAuUUGCUGuCGa-GCCCGCGCCGCu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 3008 | 0.72 | 0.545114 |
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Target: 5'- gGGaAAGCGACAGaa-GGGgGCGGCGu -3' miRNA: 3'- -UCaUUUGCUGUCgagCCCgCGCCGCu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 89376 | 0.72 | 0.545114 |
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Target: 5'- uGGgagAGAgGACAGCUUaagGGGCGUGGUGu -3' miRNA: 3'- -UCa--UUUgCUGUCGAG---CCCGCGCCGCu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 133028 | 0.75 | 0.404698 |
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Target: 5'- cGGUGGugG-CAGagUCGGGCGCGGUGc -3' miRNA: 3'- -UCAUUugCuGUCg-AGCCCGCGCCGCu -5' |
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| 13367 | 3' | -56.6 | NC_003409.1 | + | 15822 | 1.09 | 0.002397 |
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Target: 5'- uAGUAAACGACAGCUCGGGCGCGGCGAg -3' miRNA: 3'- -UCAUUUGCUGUCGAGCCCGCGCCGCU- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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