Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 1342 | 3' | -56.3 | NC_001335.1 | + | 42285 | 0.66 | 0.582378 |
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Target: 5'- cGUGACGAcGCGGCAGaggaGCUGcacgCGCUGGu -3' miRNA: 3'- -UACUGCU-UGUCGUC----CGGCa---GCGACUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 26675 | 0.66 | 0.612311 |
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Target: 5'- -cGACGGcCGGCucaAGGCCGUUcggcucgguccccgGCUGAUc -3' miRNA: 3'- uaCUGCUuGUCG---UCCGGCAG--------------CGACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 4688 | 0.66 | 0.615648 |
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Target: 5'- --cGCGAGC-GCGGGCCauGUCGUgGACc -3' miRNA: 3'- uacUGCUUGuCGUCCGG--CAGCGaCUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 29080 | 0.66 | 0.62678 |
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Target: 5'- gGUGugGAGCGGCuGaGCaagaaGCUGGCg -3' miRNA: 3'- -UACugCUUGUCGuC-CGgcag-CGACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 22971 | 0.66 | 0.62678 |
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Target: 5'- gGUGAUGccGACGGUcggAGcGuuGUCGUUGACg -3' miRNA: 3'- -UACUGC--UUGUCG---UC-CggCAGCGACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 1155 | 0.66 | 0.62678 |
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Target: 5'- -gGAuCGGGCGGCguAGGCggCGUCGgaGGCg -3' miRNA: 3'- uaCU-GCUUGUCG--UCCG--GCAGCgaCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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