Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
|
Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1342 | 3' | -56.3 | NC_001335.1 | + | 37260 | 0.67 | 0.560394 |
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Target: 5'- -gGACGAGCGGUaucccaagGGGCC-UCGCcaguggGACa -3' miRNA: 3'- uaCUGCUUGUCG--------UCCGGcAGCGa-----CUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 32102 | 0.69 | 0.432407 |
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Target: 5'- -cGGCGAcguucuacaucgagGcCAGCAGGUCGgUGCUGACc -3' miRNA: 3'- uaCUGCU--------------U-GUCGUCCGGCaGCGACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 41340 | 0.69 | 0.426592 |
|
Target: 5'- -cGaACGAGCuacGCGGGCUGUCGCgagcagaacaucgagGACg -3' miRNA: 3'- uaC-UGCUUGu--CGUCCGGCAGCGa--------------CUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 22232 | 0.7 | 0.36147 |
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Target: 5'- -cGGCGAacuucaggaACAGCGGGCCGUC-UUGGa -3' miRNA: 3'- uaCUGCU---------UGUCGUCCGGCAGcGACUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 2868 | 0.72 | 0.28209 |
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Target: 5'- uUGACGAGCugguGCGugcGGCCGUCG-UGGCu -3' miRNA: 3'- uACUGCUUGu---CGU---CCGGCAGCgACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 43735 | 0.66 | 0.631234 |
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Target: 5'- -cGGCGugguuGCcGCAGGgcaccgcguuucacaCCGUCGCUGAg -3' miRNA: 3'- uaCUGCu----UGuCGUCC---------------GGCAGCGACUg -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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