Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1342 | 3' | -56.3 | NC_001335.1 | + | 42285 | 0.66 | 0.582378 |
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Target: 5'- cGUGACGAcGCGGCAGaggaGCUGcacgCGCUGGu -3' miRNA: 3'- -UACUGCU-UGUCGUC----CGGCa---GCGACUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 42590 | 0.67 | 0.560394 |
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Target: 5'- cAUGACGAACu--GGGauccaaaccacCCGUCGCUGAg -3' miRNA: 3'- -UACUGCUUGucgUCC-----------GGCAGCGACUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 43735 | 0.66 | 0.631234 |
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Target: 5'- -cGGCGugguuGCcGCAGGgcaccgcguuucacaCCGUCGCUGAg -3' miRNA: 3'- uaCUGCu----UGuCGUCC---------------GGCAGCGACUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 45201 | 0.66 | 0.59233 |
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Target: 5'- cUGGCGGGCGGUcuccuucAGGUCGgacuUCGCcGACa -3' miRNA: 3'- uACUGCUUGUCG-------UCCGGC----AGCGaCUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 50181 | 0.69 | 0.435333 |
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Target: 5'- uUGGC--ACAGaAGGCCGUCGCguccgGGCa -3' miRNA: 3'- uACUGcuUGUCgUCCGGCAGCGa----CUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 50219 | 0.71 | 0.319939 |
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Target: 5'- uUGAcCGGAgGGCAGGCCGaugcUGUUGGCg -3' miRNA: 3'- uACU-GCUUgUCGUCCGGCa---GCGACUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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