Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1342 | 3' | -56.3 | NC_001335.1 | + | 7660 | 0.76 | 0.160956 |
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Target: 5'- -gGGCGAugAGCAGGCCcUCGCgGAg -3' miRNA: 3'- uaCUGCUugUCGUCCGGcAGCGaCUg -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 4688 | 0.66 | 0.615648 |
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Target: 5'- --cGCGAGC-GCGGGCCauGUCGUgGACc -3' miRNA: 3'- uacUGCUUGuCGUCCGG--CAGCGaCUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 4162 | 0.67 | 0.517194 |
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Target: 5'- -aGAUGGugGGCcagacggccuucGGGCCGUCccCUGACg -3' miRNA: 3'- uaCUGCUugUCG------------UCCGGCAGc-GACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 3110 | 0.68 | 0.475386 |
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Target: 5'- -aGGCGAGCGGCGGGa----GCUGAUg -3' miRNA: 3'- uaCUGCUUGUCGUCCggcagCGACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 2868 | 0.72 | 0.28209 |
|
Target: 5'- uUGACGAGCugguGCGugcGGCCGUCG-UGGCu -3' miRNA: 3'- uACUGCUUGu---CGU---CCGGCAGCgACUG- -5' |
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| 1342 | 3' | -56.3 | NC_001335.1 | + | 1155 | 0.66 | 0.62678 |
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Target: 5'- -gGAuCGGGCGGCguAGGCggCGUCGgaGGCg -3' miRNA: 3'- uaCU-GCUUGUCG--UCCG--GCAGCgaCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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