Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1343 | 5' | -58.1 | NC_001335.1 | + | 34405 | 0.66 | 0.505085 |
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Target: 5'- gGCCAg-CGGCCCGCU-GCCGUUcGCu -3' miRNA: 3'- -UGGUgaGUCGGGUGAgUGGCAGuCGc -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 19537 | 0.67 | 0.494829 |
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Target: 5'- cCCACUCGagcaCCCGCUCGCC---AGCGa -3' miRNA: 3'- uGGUGAGUc---GGGUGAGUGGcagUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 46764 | 0.67 | 0.445081 |
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Target: 5'- -aCGC-CAGCCuCACggGCgGUCAGCGg -3' miRNA: 3'- ugGUGaGUCGG-GUGagUGgCAGUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 4157 | 0.69 | 0.371723 |
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Target: 5'- cGCCGC-CGGCUgcCUCGCCgGUCGGCa -3' miRNA: 3'- -UGGUGaGUCGGguGAGUGG-CAGUCGc -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 32953 | 0.7 | 0.338262 |
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Target: 5'- gGCCccgGCUCcugAGCCUgagACcgCGCCGUCGGCGa -3' miRNA: 3'- -UGG---UGAG---UCGGG---UGa-GUGGCAGUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 12771 | 0.66 | 0.557602 |
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Target: 5'- gAUCAgaUCGGCCCggaACgUCGCCGacgCAGCGg -3' miRNA: 3'- -UGGUg-AGUCGGG---UG-AGUGGCa--GUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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