Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1343 | 5' | -58.1 | NC_001335.1 | + | 3186 | 0.66 | 0.546955 |
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Target: 5'- gACCA-UCAGCucccgCCGCUCGCC-UUGGCGa -3' miRNA: 3'- -UGGUgAGUCG-----GGUGAGUGGcAGUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 8739 | 0.66 | 0.546955 |
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Target: 5'- cACCACU--GCCacaGCUCuuCGUCAGCc -3' miRNA: 3'- -UGGUGAguCGGg--UGAGugGCAGUCGc -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 7220 | 0.66 | 0.556535 |
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Target: 5'- gGCCguACUCGGCCUucaGCuucuugcugaucaUCACCGggaaCAGCGa -3' miRNA: 3'- -UGG--UGAGUCGGG---UG-------------AGUGGCa---GUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 12771 | 0.66 | 0.557602 |
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Target: 5'- gAUCAgaUCGGCCCggaACgUCGCCGacgCAGCGg -3' miRNA: 3'- -UGGUg-AGUCGGG---UG-AGUGGCa--GUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 22666 | 0.66 | 0.557602 |
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Target: 5'- cACCGC-CGGCCa---CGCCGcCAGCGu -3' miRNA: 3'- -UGGUGaGUCGGgugaGUGGCaGUCGC- -5' |
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| 1343 | 5' | -58.1 | NC_001335.1 | + | 13081 | 0.66 | 0.557602 |
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Target: 5'- gGCCAC-C-GCCagaUCACgGUCGGCGg -3' miRNA: 3'- -UGGUGaGuCGGgugAGUGgCAGUCGC- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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