Results 1 - 20 of 31 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1345 | 5' | -62.5 | NC_001335.1 | + | 20209 | 0.66 | 0.359172 |
Target: 5'- -aGCCGuCCCgGAGCGGgaucuuCUGCAGCagguCUUCg -3' miRNA: 3'- agCGGU-GGG-CUCGCC------GACGUCG----GAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 9767 | 0.66 | 0.359172 |
Target: 5'- aCGCCgACCCGA-CGGUccCAGgCUCCc -3' miRNA: 3'- aGCGG-UGGGCUcGCCGacGUCgGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 10471 | 0.66 | 0.359172 |
Target: 5'- cUCGUCAUCgagguUGAGCc-CUGCGGCUUCCg -3' miRNA: 3'- -AGCGGUGG-----GCUCGccGACGUCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 22470 | 0.66 | 0.35105 |
Target: 5'- -aGCUGCauuCGAggcuGCGGuCUGCAGCCacUCCa -3' miRNA: 3'- agCGGUGg--GCU----CGCC-GACGUCGG--AGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 185 | 0.66 | 0.35105 |
Target: 5'- aCGCCACUccugaCGGGUGGCUGUcaaGGauaCUCa -3' miRNA: 3'- aGCGGUGG-----GCUCGCCGACG---UCg--GAGg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 32688 | 0.66 | 0.34306 |
Target: 5'- gCGCCGgCCaacGGCGGCgGCGGUggCCa -3' miRNA: 3'- aGCGGUgGGc--UCGCCGaCGUCGgaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 48759 | 0.66 | 0.34306 |
Target: 5'- aUCGaCCucgaACCCGuagcgGGCGGC-GCaAGCCUCg -3' miRNA: 3'- -AGC-GG----UGGGC-----UCGCCGaCG-UCGGAGg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 1270 | 0.66 | 0.34306 |
Target: 5'- -aGCCGgCgGcGGUGGCaGCAGCC-CCa -3' miRNA: 3'- agCGGUgGgC-UCGCCGaCGUCGGaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 31505 | 0.66 | 0.327476 |
Target: 5'- cUCGCCAgCCaCGAcGUGGaaGCGGuCCUCg -3' miRNA: 3'- -AGCGGU-GG-GCU-CGCCgaCGUC-GGAGg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 28576 | 0.66 | 0.319882 |
Target: 5'- cUCGUCGCCUGcgggaaGGUGGCgcacuCGGCgCUCCa -3' miRNA: 3'- -AGCGGUGGGC------UCGCCGac---GUCG-GAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 39233 | 0.67 | 0.283905 |
Target: 5'- uUCGCCGCCCu--CGGCacgcGCGuCCUCCu -3' miRNA: 3'- -AGCGGUGGGcucGCCGa---CGUcGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 6983 | 0.67 | 0.283905 |
Target: 5'- gCGCCugCUuGGCGGaacuCUGUGGCC-CCg -3' miRNA: 3'- aGCGGugGGcUCGCC----GACGUCGGaGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 18052 | 0.67 | 0.283219 |
Target: 5'- -gGCCggcgaagACCCGu-UGGUgaagGCAGCCUCCg -3' miRNA: 3'- agCGG-------UGGGCucGCCGa---CGUCGGAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 25827 | 0.68 | 0.270436 |
Target: 5'- uUCGCCcugucguucacgGCUcucagCGAGCuGGCUGCGGCCa-- -3' miRNA: 3'- -AGCGG------------UGG-----GCUCG-CCGACGUCGGagg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 3103 | 0.68 | 0.263897 |
Target: 5'- cUCGCCAaggCGAGCGGCgGgAGCUgauggUCCc -3' miRNA: 3'- -AGCGGUgg-GCUCGCCGaCgUCGG-----AGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 1148 | 0.68 | 0.263897 |
Target: 5'- -aGCC-CCCGgaucgGGCGGCguagGCGGCgUCg -3' miRNA: 3'- agCGGuGGGC-----UCGCCGa---CGUCGgAGg -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 19160 | 0.68 | 0.257486 |
Target: 5'- cUCGCCGuagaucaacCCCGgcgucuugagccGGCGGUUGCGGaaCUCCu -3' miRNA: 3'- -AGCGGU---------GGGC------------UCGCCGACGUCg-GAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 5604 | 0.68 | 0.257486 |
Target: 5'- gCGCCGgCCGuAGCGGCaggcaccguacGCAGCCagCg -3' miRNA: 3'- aGCGGUgGGC-UCGCCGa----------CGUCGGagG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 25198 | 0.68 | 0.239018 |
Target: 5'- cUCGCC-CgCGAGCcaGGCUuCGGCgUCCa -3' miRNA: 3'- -AGCGGuGgGCUCG--CCGAcGUCGgAGG- -5' |
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1345 | 5' | -62.5 | NC_001335.1 | + | 829 | 0.68 | 0.239018 |
Target: 5'- gCGCCACaCC-AGCGGgaGCGGUagacgUUCCa -3' miRNA: 3'- aGCGGUG-GGcUCGCCgaCGUCG-----GAGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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