Results 21 - 26 of 26 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13453 | 5' | -50.1 | NC_003409.1 | + | 118301 | 0.73 | 0.780612 |
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Target: 5'- cCGGGGAGGAGgcuggGGUGcGCCgcgccgGGUu -3' miRNA: 3'- -GCCUCUCCUUa----CCAC-CGGa-----CCA- -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 12194 | 0.73 | 0.760252 |
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Target: 5'- cCGGAGAGGAcgGaGUGGCCc--- -3' miRNA: 3'- -GCCUCUCCUuaC-CACCGGacca -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 22757 | 0.73 | 0.746726 |
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Target: 5'- uGGGGAGGAAaagcgcggcaccccUGGUcGCCUGGg -3' miRNA: 3'- gCCUCUCCUU--------------ACCAcCGGACCa -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 87917 | 0.76 | 0.61907 |
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Target: 5'- gGGAGAucuuccugucacGGGAUGGUGGCCUcaGGg -3' miRNA: 3'- gCCUCU------------CCUUACCACCGGA--CCa -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 90959 | 0.76 | 0.585917 |
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Target: 5'- uGGGGAGGggUGG-GGCaaaUGGUu -3' miRNA: 3'- gCCUCUCCuuACCaCCGg--ACCA- -5' |
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| 13453 | 5' | -50.1 | NC_003409.1 | + | 117611 | 1.03 | 0.014084 |
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Target: 5'- gCGGAGAGGAAUGGUGGCCUGGUu -3' miRNA: 3'- -GCCUCUCCUUACCACCGGACCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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