Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1357 | 3' | -56.5 | NC_001335.1 | + | 32612 | 0.66 | 0.625395 |
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Target: 5'- aGAAG-CGGUacuGCCaGCA-CGGCGAGAUg -3' miRNA: 3'- -CUUCaGUCG---CGGcCGUaGUCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 32890 | 0.66 | 0.614304 |
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Target: 5'- aGGAGcCGGgGCCGGC-UCGgGCuGGGCa -3' miRNA: 3'- -CUUCaGUCgCGGCCGuAGU-CGcUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 34271 | 0.67 | 0.505698 |
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Target: 5'- -cGGUguGCGCCGGU--CGGCGcGGAUg -3' miRNA: 3'- cuUCAguCGCGGCCGuaGUCGC-UCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 37527 | 0.67 | 0.52689 |
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Target: 5'- cGAGGaUCGGCuuGUgGGUGUCAGUGAcGACg -3' miRNA: 3'- -CUUC-AGUCG--CGgCCGUAGUCGCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 39607 | 0.68 | 0.495239 |
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Target: 5'- -cAGUCGGCacCCGGCAcugCAuGCGAGAg -3' miRNA: 3'- cuUCAGUCGc-GGCCGUa--GU-CGCUCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 41488 | 0.67 | 0.505698 |
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Target: 5'- -uGGUCuGCGCCGGCccCGGCaccGGGAa -3' miRNA: 3'- cuUCAGuCGCGGCCGuaGUCG---CUCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 46346 | 0.66 | 0.581162 |
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Target: 5'- -cGGUCAGCGCgaCGGCcgCAacGCGGcGAUg -3' miRNA: 3'- cuUCAGUCGCG--GCCGuaGU--CGCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 52092 | 0.68 | 0.48488 |
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Target: 5'- --uGUCAGCGCgGGUAUgGGCGuGuauCg -3' miRNA: 3'- cuuCAGUCGCGgCCGUAgUCGCuCu--G- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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