Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1357 | 3' | -56.5 | NC_001335.1 | + | 2315 | 0.67 | 0.54516 |
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Target: 5'- cGAGGUCGGCaaggagugagcuggGCCGGC-UCAGgcCGGcGACa -3' miRNA: 3'- -CUUCAGUCG--------------CGGCCGuAGUC--GCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 16001 | 0.66 | 0.57019 |
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Target: 5'- uGAAGUCGGCcuugGCCugGGCcgCAuCGAGAUg -3' miRNA: 3'- -CUUCAGUCG----CGG--CCGuaGUcGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 5613 | 0.66 | 0.57019 |
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Target: 5'- cGAGGUCAGcCGCCGaC-UCAGCGucGAg -3' miRNA: 3'- -CUUCAGUC-GCGGCcGuAGUCGCu-CUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 3093 | 0.66 | 0.580063 |
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Target: 5'- cAAG-CAGCGCCucgccaaGGCGagCGGCGGGAg -3' miRNA: 3'- cUUCaGUCGCGG-------CCGUa-GUCGCUCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 46346 | 0.66 | 0.581162 |
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Target: 5'- -cGGUCAGCGCgaCGGCcgCAacGCGGcGAUg -3' miRNA: 3'- cuUCAGUCGCG--GCCGuaGU--CGCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 29861 | 0.66 | 0.585564 |
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Target: 5'- uGAGUCGGuCGCUGGUgccguacgagcacaaGaUCAGUGAGAUc -3' miRNA: 3'- cUUCAGUC-GCGGCCG---------------U-AGUCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 32890 | 0.66 | 0.614304 |
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Target: 5'- aGGAGcCGGgGCCGGC-UCGgGCuGGGCa -3' miRNA: 3'- -CUUCaGUCgCGGCCGuAGU-CGcUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 32612 | 0.66 | 0.625395 |
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Target: 5'- aGAAG-CGGUacuGCCaGCA-CGGCGAGAUg -3' miRNA: 3'- -CUUCaGUCG---CGGcCGUaGUCGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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