Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1357 | 3' | -56.5 | NC_001335.1 | + | 34271 | 0.67 | 0.505698 |
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Target: 5'- -cGGUguGCGCCGGU--CGGCGcGGAUg -3' miRNA: 3'- cuUCAguCGCGGCCGuaGUCGC-UCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 41488 | 0.67 | 0.505698 |
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Target: 5'- -uGGUCuGCGCCGGCccCGGCaccGGGAa -3' miRNA: 3'- cuUCAGuCGCGGCCGuaGUCG---CUCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 39607 | 0.68 | 0.495239 |
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Target: 5'- -cAGUCGGCacCCGGCAcugCAuGCGAGAg -3' miRNA: 3'- cuUCAGUCGc-GGCCGUa--GU-CGCUCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 3605 | 0.68 | 0.48488 |
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Target: 5'- uGAAGuUCAGUGUCcacGCAgggaaCAGCGAGACc -3' miRNA: 3'- -CUUC-AGUCGCGGc--CGUa----GUCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 20628 | 0.69 | 0.448487 |
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Target: 5'- gGAGGUCAaucgcuccaccgaccGCGCCGaGCG-CGGCGAG-Cu -3' miRNA: 3'- -CUUCAGU---------------CGCGGC-CGUaGUCGCUCuG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 16665 | 0.69 | 0.415548 |
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Target: 5'- cGAGUUcGCGCCGGCGUuggaCAGCGucaGGAa -3' miRNA: 3'- cUUCAGuCGCGGCCGUA----GUCGC---UCUg -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 16322 | 0.7 | 0.367278 |
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Target: 5'- --cGcCAGCGCCGGCuccgaacgcagacagCAGCGAcGGCa -3' miRNA: 3'- cuuCaGUCGCGGCCGua-------------GUCGCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 32612 | 0.66 | 0.625395 |
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Target: 5'- aGAAG-CGGUacuGCCaGCA-CGGCGAGAUg -3' miRNA: 3'- -CUUCaGUCG---CGGcCGUaGUCGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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