Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1357 | 3' | -56.5 | NC_001335.1 | + | 16322 | 0.7 | 0.367278 |
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Target: 5'- --cGcCAGCGCCGGCuccgaacgcagacagCAGCGAcGGCa -3' miRNA: 3'- cuuCaGUCGCGGCCGua-------------GUCGCU-CUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 27404 | 0.7 | 0.361197 |
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Target: 5'- uGAAGUCGGCGUCuGCcUCggagaGGUGGGACg -3' miRNA: 3'- -CUUCAGUCGCGGcCGuAG-----UCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 17573 | 0.71 | 0.327812 |
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Target: 5'- --cGUcCAGgGCCugaucaacGGCAUCGGCGGGAUg -3' miRNA: 3'- cuuCA-GUCgCGG--------CCGUAGUCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 11904 | 0.72 | 0.29602 |
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Target: 5'- uGAGGUCGGCguuggaGCCGGUgccguccggguccAcCAGCGAGACc -3' miRNA: 3'- -CUUCAGUCG------CGGCCG-------------UaGUCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 5593 | 0.72 | 0.268053 |
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Target: 5'- -uAGUCuccgguGCGCCGGCcgUAGCGgcAGGCa -3' miRNA: 3'- cuUCAGu-----CGCGGCCGuaGUCGC--UCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 8720 | 0.74 | 0.206091 |
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Target: 5'- --cGUCAGCcucGUCGGCAUCGcCGAGGCg -3' miRNA: 3'- cuuCAGUCG---CGGCCGUAGUcGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 17218 | 0.75 | 0.165816 |
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Target: 5'- cGAGGgccgucgcCAGCGCCGGgAUguGCGGGGCc -3' miRNA: 3'- -CUUCa-------GUCGCGGCCgUAguCGCUCUG- -5' |
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| 1357 | 3' | -56.5 | NC_001335.1 | + | 16917 | 1.1 | 0.00049 |
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Target: 5'- cGAAGUCAGCGCCGGCAUCAGCGAGACg -3' miRNA: 3'- -CUUCAGUCGCGGCCGUAGUCGCUCUG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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