Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1363 | 5' | -62 | NC_001335.1 | + | 32664 | 0.68 | 0.266449 |
Target: 5'- gGCCAGcagcagucucgcGCCcCGCAGGCagcucaGGAAG-CGCCu -3' miRNA: 3'- -UGGUC------------CGGcGCGUCCG------CCUUCaGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 12185 | 0.69 | 0.247412 |
Target: 5'- gACCuGGCCccagacgaGCuGGCGGAAGUCaCCc -3' miRNA: 3'- -UGGuCCGGcg------CGuCCGCCUUCAGcGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 5957 | 0.69 | 0.241321 |
Target: 5'- gACCAGGCCcgaGCGGGuCGGGcuGGccugUGCCg -3' miRNA: 3'- -UGGUCCGGcg-CGUCC-GCCU--UCa---GCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 15490 | 0.69 | 0.237727 |
Target: 5'- -aCAGGUCGCGCAGGUcuacccgguaguacuGGAgcaggucugcgaGGaUCGCCc -3' miRNA: 3'- ugGUCCGGCGCGUCCG---------------CCU------------UC-AGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 612 | 0.7 | 0.197 |
Target: 5'- uCCAGGCCGCGagaUAGGCcggcacGAAGUCggucgGCCg -3' miRNA: 3'- uGGUCCGGCGC---GUCCGc-----CUUCAG-----CGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 453 | 0.71 | 0.167259 |
Target: 5'- cACCccucuauaGGGCCGCGCAcuGGCGcGGccuugagauccggcGGUUGCCg -3' miRNA: 3'- -UGG--------UCCGGCGCGU--CCGC-CU--------------UCAGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 1701 | 0.71 | 0.167259 |
Target: 5'- aACCAGGCguccucgaggagggCGCGUucGGuCGGGGuGUCGCCa -3' miRNA: 3'- -UGGUCCG--------------GCGCGu-CC-GCCUU-CAGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 46197 | 0.75 | 0.093134 |
Target: 5'- uACCGGaGCgcaGCGCAcaucgccgagcacGGCGGAaaAGUCGCCg -3' miRNA: 3'- -UGGUC-CGg--CGCGU-------------CCGCCU--UCAGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 25268 | 0.66 | 0.370939 |
Target: 5'- aGCCuGGCU-CGCGGGCGaGAAG-CGgCu -3' miRNA: 3'- -UGGuCCGGcGCGUCCGC-CUUCaGCgG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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