Results 21 - 29 of 29 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
1363 | 5' | -62 | NC_001335.1 | + | 36742 | 0.66 | 0.346361 |
Target: 5'- aACCGGGCCGuCGUAGaccuggcgcaGCGGcuccAGgaacCGCCg -3' miRNA: 3'- -UGGUCCGGC-GCGUC----------CGCCu---UCa---GCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 38324 | 0.66 | 0.353611 |
Target: 5'- gGCUGGGCuucCGgGCAGGCuuucucuguaccgGGAcGUUGCCu -3' miRNA: 3'- -UGGUCCG---GCgCGUCCG-------------CCUuCAGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 16252 | 0.66 | 0.354423 |
Target: 5'- uUCGGaGCCgGCGCuGGCGu-AGUCGCg -3' miRNA: 3'- uGGUC-CGG-CGCGuCCGCcuUCAGCGg -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 46250 | 0.66 | 0.36179 |
Target: 5'- cGCCAGcgcgugguucaucGCCGCGUu-GCGGccGUCGCg -3' miRNA: 3'- -UGGUC-------------CGGCGCGucCGCCuuCAGCGg -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 7686 | 0.66 | 0.362615 |
Target: 5'- uAUC-GGCaGC-CAGGUGGA-GUCGCCu -3' miRNA: 3'- -UGGuCCGgCGcGUCCGCCUuCAGCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 10253 | 0.66 | 0.362615 |
Target: 5'- gGCCAGaGCCaacuuagagGuCGCAcucGGCGGAgAGUCgGCCu -3' miRNA: 3'- -UGGUC-CGG---------C-GCGU---CCGCCU-UCAG-CGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 6976 | 0.66 | 0.370939 |
Target: 5'- cACC--GCCGCGCcugcuuGGCGGAAcucuGUgGCCc -3' miRNA: 3'- -UGGucCGGCGCGu-----CCGCCUU----CAgCGG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 25268 | 0.66 | 0.370939 |
Target: 5'- aGCCuGGCU-CGCGGGCGaGAAG-CGgCu -3' miRNA: 3'- -UGGuCCGGcGCGUCCGC-CUUCaGCgG- -5' |
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1363 | 5' | -62 | NC_001335.1 | + | 10259 | 0.66 | 0.382809 |
Target: 5'- gAUCcGGCCcCGCagaucgugcagggcuGGGCGaGGGUCGCCa -3' miRNA: 3'- -UGGuCCGGcGCG---------------UCCGCcUUCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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