Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1363 | 5' | -62 | NC_001335.1 | + | 32903 | 0.7 | 0.202126 |
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Target: 5'- gGCuCGGGCUGgGCagGGGCGGugcuccacGGUCGUCg -3' miRNA: 3'- -UG-GUCCGGCgCG--UCCGCCu-------UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 35864 | 0.67 | 0.308046 |
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Target: 5'- --gAGGCUcUGCAGG-GGGAGUCGaCCa -3' miRNA: 3'- uggUCCGGcGCGUCCgCCUUCAGC-GG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 36742 | 0.66 | 0.346361 |
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Target: 5'- aACCGGGCCGuCGUAGaccuggcgcaGCGGcuccAGgaacCGCCg -3' miRNA: 3'- -UGGUCCGGC-GCGUC----------CGCCu---UCa---GCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 38324 | 0.66 | 0.353611 |
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Target: 5'- gGCUGGGCuucCGgGCAGGCuuucucuguaccgGGAcGUUGCCu -3' miRNA: 3'- -UGGUCCG---GCgCGUCCG-------------CCUuCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 42399 | 0.72 | 0.139732 |
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Target: 5'- aGCCGGguGCCGCGCAGcuugaGGAucuucauGGUCGCCc -3' miRNA: 3'- -UGGUC--CGGCGCGUCcg---CCU-------UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 46197 | 0.75 | 0.093134 |
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Target: 5'- uACCGGaGCgcaGCGCAcaucgccgagcacGGCGGAaaAGUCGCCg -3' miRNA: 3'- -UGGUC-CGg--CGCGU-------------CCGCCU--UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 46250 | 0.66 | 0.36179 |
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Target: 5'- cGCCAGcgcgugguucaucGCCGCGUu-GCGGccGUCGCg -3' miRNA: 3'- -UGGUC-------------CGGCGCGucCGCCuuCAGCGg -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 48353 | 0.67 | 0.293651 |
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Target: 5'- gGCC-GGCCGCGaCAagguacGGCGcGAG-CGCCg -3' miRNA: 3'- -UGGuCCGGCGC-GU------CCGCcUUCaGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 50844 | 0.73 | 0.11928 |
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Target: 5'- aGCCAGGCCGa-CAGGCcGGgcGUCaCCg -3' miRNA: 3'- -UGGUCCGGCgcGUCCG-CCuuCAGcGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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