Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1363 | 5' | -62 | NC_001335.1 | + | 1701 | 0.71 | 0.167259 |
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Target: 5'- aACCAGGCguccucgaggagggCGCGUucGGuCGGGGuGUCGCCa -3' miRNA: 3'- -UGGUCCG--------------GCGCGu-CC-GCCUU-CAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 453 | 0.71 | 0.167259 |
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Target: 5'- cACCccucuauaGGGCCGCGCAcuGGCGcGGccuugagauccggcGGUUGCCg -3' miRNA: 3'- -UGG--------UCCGGCGCGU--CCGC-CU--------------UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 42399 | 0.72 | 0.139732 |
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Target: 5'- aGCCGGguGCCGCGCAGcuugaGGAucuucauGGUCGCCc -3' miRNA: 3'- -UGGUC--CGGCGCGUCcg---CCU-------UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 1156 | 0.73 | 0.129306 |
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Target: 5'- gAUCGGGCgGCGUAGGCGGc-GUCGg- -3' miRNA: 3'- -UGGUCCGgCGCGUCCGCCuuCAGCgg -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 50844 | 0.73 | 0.11928 |
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Target: 5'- aGCCAGGCCGa-CAGGCcGGgcGUCaCCg -3' miRNA: 3'- -UGGUCCGGCgcGUCCG-CCuuCAGcGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 46197 | 0.75 | 0.093134 |
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Target: 5'- uACCGGaGCgcaGCGCAcaucgccgagcacGGCGGAaaAGUCGCCg -3' miRNA: 3'- -UGGUC-CGg--CGCGU-------------CCGCCU--UCAGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 5708 | 0.77 | 0.065227 |
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Target: 5'- gGCCAGGCagaGUGCcgAGGCuGAAGUCGCg -3' miRNA: 3'- -UGGUCCGg--CGCG--UCCGcCUUCAGCGg -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 9660 | 0.84 | 0.017298 |
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Target: 5'- gGCCuuGGCCGCGUAGGUGGGAGugcucgggucUCGCCa -3' miRNA: 3'- -UGGu-CCGGCGCGUCCGCCUUC----------AGCGG- -5' |
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| 1363 | 5' | -62 | NC_001335.1 | + | 19512 | 1.11 | 0.000147 |
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Target: 5'- gACCAGGCCGCGCAGGCGGAAGUCGCCa -3' miRNA: 3'- -UGGUCCGGCGCGUCCGCCUUCAGCGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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