Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand |
Start Position
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R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1364 | 5' | -55.6 | NC_001335.1 | + | 9888 | 0.68 | 0.545197 |
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Target: 5'- cGACuCGAUggCCGGcuguggcUUCGGCGGUGCCg -3' miRNA: 3'- -CUGcGCUAgaGGCU-------AGGUCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 9270 | 0.66 | 0.688533 |
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Target: 5'- gGACGCuGAgggcaaGAUCCAGCAGUucucugcaGCCg -3' miRNA: 3'- -CUGCG-CUagagg-CUAGGUCGUCA--------CGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 8491 | 0.75 | 0.216143 |
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Target: 5'- uGCGgGGUCUUCGAUCUccuccauaccggggAGCGGUGCUg -3' miRNA: 3'- cUGCgCUAGAGGCUAGG--------------UCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 5870 | 0.67 | 0.644688 |
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Target: 5'- cACGUugagGAUCUgCGuggCCAGCGG-GCCg -3' miRNA: 3'- cUGCG----CUAGAgGCua-GGUCGUCaCGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 5584 | 0.69 | 0.524935 |
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Target: 5'- -cCGCcccaGAUCaggaCGAUgcCCAGCAGUGCCg -3' miRNA: 3'- cuGCG----CUAGag--GCUA--GGUCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 4813 | 0.67 | 0.622654 |
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Target: 5'- ---aCGGUUUCCGAcCCAGuCGGUGCg -3' miRNA: 3'- cugcGCUAGAGGCUaGGUC-GUCACGg -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 1823 | 0.69 | 0.493555 |
|
Target: 5'- uGACGCGGUCcuugaCCGAgUCCuucauguagAGCuuGGUGCCg -3' miRNA: 3'- -CUGCGCUAGa----GGCU-AGG---------UCG--UCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 1302 | 0.7 | 0.433587 |
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Target: 5'- uGACGC---CUCCGGUaCCGGCGcUGCCg -3' miRNA: 3'- -CUGCGcuaGAGGCUA-GGUCGUcACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 430 | 0.77 | 0.175823 |
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Target: 5'- uGGCGCGGcCUUgaGAUCCGGCGGUuGCCg -3' miRNA: 3'- -CUGCGCUaGAGg-CUAGGUCGUCA-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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