Results 21 - 29 of 29 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1364 | 5' | -55.6 | NC_001335.1 | + | 9270 | 0.66 | 0.688533 |
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Target: 5'- gGACGCuGAgggcaaGAUCCAGCAGUucucugcaGCCg -3' miRNA: 3'- -CUGCG-CUagagg-CUAGGUCGUCA--------CGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 46493 | 0.66 | 0.655692 |
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Target: 5'- cGACcgGCGGUagCUCuUGAgcuggCCAGCGGUGCUc -3' miRNA: 3'- -CUG--CGCUA--GAG-GCUa----GGUCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 12611 | 0.67 | 0.622654 |
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Target: 5'- uGGCGCuGAUCgagcgCCGGcUCCAGCAGaucgaGCg -3' miRNA: 3'- -CUGCG-CUAGa----GGCU-AGGUCGUCa----CGg -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 40654 | 0.69 | 0.473119 |
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Target: 5'- cACGCGGagaggcccaggaUCgCCGAUCCGGCgaugAGUGCg -3' miRNA: 3'- cUGCGCU------------AGaGGCUAGGUCG----UCACGg -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 50778 | 0.7 | 0.453118 |
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Target: 5'- gGGCGCGuuggCUCgCGGUgCCuuacggauGGCGGUGCCu -3' miRNA: 3'- -CUGCGCua--GAG-GCUA-GG--------UCGUCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 29376 | 0.7 | 0.453118 |
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Target: 5'- uGCGCGAggUCCGcgCCuGCAGUGg- -3' miRNA: 3'- cUGCGCUagAGGCuaGGuCGUCACgg -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 11465 | 0.71 | 0.396042 |
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Target: 5'- aGCGgagaaGAUCUCCaucGUCCAGCAGUucGCCc -3' miRNA: 3'- cUGCg----CUAGAGGc--UAGGUCGUCA--CGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 13003 | 0.72 | 0.352163 |
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Target: 5'- uGACuGCGAUCacgaagCUGAUCCuccGguGUGCCg -3' miRNA: 3'- -CUG-CGCUAGa-----GGCUAGGu--CguCACGG- -5' |
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| 1364 | 5' | -55.6 | NC_001335.1 | + | 40503 | 0.66 | 0.710174 |
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Target: 5'- cGACGCGGUgUCCGcguugAUUgAGCAccgGCCc -3' miRNA: 3'- -CUGCGCUAgAGGC-----UAGgUCGUca-CGG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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