Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. |
strand
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Start Position | R_P_ratio# | P value |
| Predicted miRNA align pattern | |||||||
| 1369 | 3' | -55.8 | NC_001335.1 | + | 5970 | 0.67 | 0.590928 |
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Target: 5'- --cGGCAGCGgCGuucccgauuGGCCGU-CGGUCg -3' miRNA: 3'- caaCCGUUGCgGUu--------CUGGCAgGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 29341 | 0.67 | 0.579956 |
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Target: 5'- --cGGCAGCGUCugcGCCGggaugCCGGUg -3' miRNA: 3'- caaCCGUUGCGGuucUGGCa----GGCCAg -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 39787 | 0.68 | 0.558151 |
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Target: 5'- -gUGGcCAACGCUucccAGACCGUggugCGGUCc -3' miRNA: 3'- caACC-GUUGCGGu---UCUGGCAg---GCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 15895 | 0.69 | 0.453781 |
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Target: 5'- --gGGCGAUGUCGAGAUCaa-CGGUCa -3' miRNA: 3'- caaCCGUUGCGGUUCUGGcagGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 14752 | 0.7 | 0.443911 |
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Target: 5'- -cUGGCuGCGCU--GcCCGUCCGGUUc -3' miRNA: 3'- caACCGuUGCGGuuCuGGCAGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 24089 | 0.7 | 0.434164 |
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Target: 5'- -cUGGCGGCGgCGGGGCUGgugCCcaGGUCu -3' miRNA: 3'- caACCGUUGCgGUUCUGGCa--GG--CCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 1225 | 0.7 | 0.396458 |
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Target: 5'- --cGGCAGCGCCGGuACCGgaggcgucaCCGGUg -3' miRNA: 3'- caaCCGUUGCGGUUcUGGCa--------GGCCAg -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 49536 | 0.66 | 0.635076 |
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Target: 5'- cUUGGUAA--CCAAGACCaaagGUCuCGGUCa -3' miRNA: 3'- cAACCGUUgcGGUUCUGG----CAG-GCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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