Results 21 - 28 of 28 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 1369 | 3' | -55.8 | NC_001335.1 | + | 9088 | 0.7 | 0.443912 |
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Target: 5'- --cGGCAcaacgggaacgACGCCcAGACCGUgCGGg- -3' miRNA: 3'- caaCCGU-----------UGCGGuUCUGGCAgGCCag -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 14752 | 0.7 | 0.443911 |
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Target: 5'- -cUGGCuGCGCU--GcCCGUCCGGUUc -3' miRNA: 3'- caACCGuUGCGGuuCuGGCAGGCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 24089 | 0.7 | 0.434164 |
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Target: 5'- -cUGGCGGCGgCGGGGCUGgugCCcaGGUCu -3' miRNA: 3'- caACCGUUGCgGUUCUGGCa--GG--CCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 9851 | 0.7 | 0.396458 |
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Target: 5'- --gGGCucggggaGCCuGGGACCGUCgGGUCg -3' miRNA: 3'- caaCCGuug----CGG-UUCUGGCAGgCCAG- -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 1225 | 0.7 | 0.396458 |
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Target: 5'- --cGGCAGCGCCGGuACCGgaggcgucaCCGGUg -3' miRNA: 3'- caaCCGUUGCGGUUcUGGCa--------GGCCAg -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 2468 | 0.73 | 0.268131 |
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Target: 5'- aGUUGGCAGCaGaaAGGACCGggagCCGGUa -3' miRNA: 3'- -CAACCGUUG-CggUUCUGGCa---GGCCAg -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 24495 | 0.75 | 0.195576 |
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Target: 5'- -gUGGCuACGCCAAGAUCGgucggCCGGa- -3' miRNA: 3'- caACCGuUGCGGUUCUGGCa----GGCCag -5' |
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| 1369 | 3' | -55.8 | NC_001335.1 | + | 24677 | 1.1 | 0.000638 |
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Target: 5'- cGUUGGCAACGCCAAGACCGUCCGGUCg -3' miRNA: 3'- -CAACCGUUGCGGUUCUGGCAGGCCAG- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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