Results 1 - 20 of 28 are showing below:
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ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# | P value |
Predicted miRNA align pattern | |||||||
13953 | 3' | -48.2 | NC_003521.1 | + | 197132 | 0.66 | 0.999973 |
Target: 5'- cCCGcGGUGCCGuugcUGGAGGugGa---- -3' miRNA: 3'- -GGCaCUACGGCu---ACUUCCugUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 225246 | 0.66 | 0.999973 |
Target: 5'- gCGUGGUGCUGGUGGGcGACcg--CUa -3' miRNA: 3'- gGCACUACGGCUACUUcCUGuaaaGA- -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 211623 | 0.66 | 0.999964 |
Target: 5'- aCGUGAUGUUcGUGAAGu-CGUUUCa -3' miRNA: 3'- gGCACUACGGcUACUUCcuGUAAAGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 111614 | 0.66 | 0.999936 |
Target: 5'- gCCGgGAU-CCGAggaGAAGGACAUggCg -3' miRNA: 3'- -GGCaCUAcGGCUa--CUUCCUGUAaaGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 175411 | 0.66 | 0.999936 |
Target: 5'- gUGUGAUGagaaccCCGG-GAAGGACAU-UCUg -3' miRNA: 3'- gGCACUAC------GGCUaCUUCCUGUAaAGA- -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 30340 | 0.66 | 0.999936 |
Target: 5'- aCCGUGAgcgGCCGgcG-GGGACGg---- -3' miRNA: 3'- -GGCACUa--CGGCuaCuUCCUGUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 148940 | 0.66 | 0.999936 |
Target: 5'- gCCGUGcuggcgcguGUGCUGcUGGAGGGCAg---- -3' miRNA: 3'- -GGCAC---------UACGGCuACUUCCUGUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 29385 | 0.66 | 0.999936 |
Target: 5'- aCCGcGAgGCCGA-GGAGGACGa---- -3' miRNA: 3'- -GGCaCUaCGGCUaCUUCCUGUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 85478 | 0.67 | 0.999925 |
Target: 5'- gUCGUGGUGCCGGUGcccgcgcccccgccGGGACccgcggUCg -3' miRNA: 3'- -GGCACUACGGCUACu-------------UCCUGuaa---AGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 87363 | 0.67 | 0.999916 |
Target: 5'- cCCGagGAggagGCCGAggUGGAGGACGa---- -3' miRNA: 3'- -GGCa-CUa---CGGCU--ACUUCCUGUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 191082 | 0.67 | 0.999891 |
Target: 5'- aCCGUGGUGgCGgcGgcGGGCugcgUCUc -3' miRNA: 3'- -GGCACUACgGCuaCuuCCUGuaa-AGA- -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 45739 | 0.67 | 0.999891 |
Target: 5'- gCCGUGGUgguggGCCgGGUGAAGGugAc---- -3' miRNA: 3'- -GGCACUA-----CGG-CUACUUCCugUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 155721 | 0.67 | 0.99986 |
Target: 5'- gCCGcUGccgccGCCGAUGggGGA-GUUUCg -3' miRNA: 3'- -GGC-ACua---CGGCUACuuCCUgUAAAGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 119401 | 0.67 | 0.99986 |
Target: 5'- gCCGUGAUGCgCGGUGAGGuauccgaguuccGACcccagUCg -3' miRNA: 3'- -GGCACUACG-GCUACUUC------------CUGuaa--AGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 127177 | 0.67 | 0.999821 |
Target: 5'- gCUGcUGAgcgaGCUGGUGGAGGACA--UCg -3' miRNA: 3'- -GGC-ACUa---CGGCUACUUCCUGUaaAGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 112743 | 0.68 | 0.999638 |
Target: 5'- gCGUGAUGCCGAaGuAGGugGc---- -3' miRNA: 3'- gGCACUACGGCUaCuUCCugUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 37479 | 0.69 | 0.998749 |
Target: 5'- aCCGUGGUGCCGc---AGGGCAc---- -3' miRNA: 3'- -GGCACUACGGCuacuUCCUGUaaaga -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 172552 | 0.7 | 0.997843 |
Target: 5'- cCUGgugGAUGCUGGggauguUGAAGGGCAggUUCUu -3' miRNA: 3'- -GGCa--CUACGGCU------ACUUCCUGUa-AAGA- -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 62605 | 0.7 | 0.997843 |
Target: 5'- aCCGUGcUGCCGA---AGGACGagUCg -3' miRNA: 3'- -GGCACuACGGCUacuUCCUGUaaAGa -5' |
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13953 | 3' | -48.2 | NC_003521.1 | + | 152481 | 0.7 | 0.996975 |
Target: 5'- gCGUGAUGgacuucaCGGUGAAGGACGa---- -3' miRNA: 3'- gGCACUACg------GCUACUUCCUGUaaaga -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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