Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position |
R_P_ratio #
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P value |
| Predicted miRNA align pattern | |||||||
| 13954 | 3' | -53.3 | NC_003521.1 | + | 160286 | 0.7 | 0.928834 |
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Target: 5'- -aGCCGucgUCGUCGuacucGUCGUCGCUGc -3' miRNA: 3'- ggCGGCua-AGUAGCc----UAGCAGCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 162298 | 0.71 | 0.918185 |
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Target: 5'- aCGCCGGgagUCcgCGGAauccgCGUCGCgUGUc -3' miRNA: 3'- gGCGGCUa--AGuaGCCUa----GCAGCG-ACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 1327 | 0.71 | 0.923622 |
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Target: 5'- cCCGCCGAUg-GUCGG-UCGa-GCUGUa -3' miRNA: 3'- -GGCGGCUAagUAGCCuAGCagCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 100816 | 0.72 | 0.892894 |
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Target: 5'- aCGCCGAgggcgaguaucgUCGUCGGAUCcccaGCUGUc -3' miRNA: 3'- gGCGGCUa-----------AGUAGCCUAGcag-CGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 89999 | 0.76 | 0.680382 |
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Target: 5'- aCCGCCGAccCcUCGGGUCGggcCGCUGg -3' miRNA: 3'- -GGCGGCUaaGuAGCCUAGCa--GCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 187766 | 0.77 | 0.660555 |
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Target: 5'- -aGCCGucUCGUCGGAUCGUCGUg-- -3' miRNA: 3'- ggCGGCuaAGUAGCCUAGCAGCGaca -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 196973 | 0.77 | 0.620702 |
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Target: 5'- gCCGCCGGUgaaGUCGGc-CGUCGCUGc -3' miRNA: 3'- -GGCGGCUAag-UAGCCuaGCAGCGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 24593 | 1.1 | 0.008639 |
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Target: 5'- gCCGCCGAUUCAUCGGAUCGUCGCUGUg -3' miRNA: 3'- -GGCGGCUAAGUAGCCUAGCAGCGACA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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