Results 41 - 48 of 48 are showing below:
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| ID | Location | Perfect MFE.* | RefSeq Acc. | strand | Start Position | R_P_ratio# |
P value
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| Predicted miRNA align pattern | |||||||
| 13954 | 3' | -53.3 | NC_003521.1 | + | 74143 | 0.66 | 0.993685 |
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Target: 5'- uUGCgGAUcUCGggCGGGgggaggcucUCGUCGCUGUg -3' miRNA: 3'- gGCGgCUA-AGUa-GCCU---------AGCAGCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 31363 | 0.66 | 0.993685 |
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Target: 5'- gCGCCGcgUgUAcCGGccgccgccGUCGUCGCUGUu -3' miRNA: 3'- gGCGGCuaA-GUaGCC--------UAGCAGCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 105194 | 0.66 | 0.994505 |
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Target: 5'- cCCGCCGA-UCGUgGGAUCcgcccgcgaGCUGc -3' miRNA: 3'- -GGCGGCUaAGUAgCCUAGcag------CGACa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 82527 | 0.66 | 0.994505 |
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Target: 5'- cCUGCUGAgUCGUcCGGGUgGUCGUa-- -3' miRNA: 3'- -GGCGGCUaAGUA-GCCUAgCAGCGaca -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 239527 | 0.66 | 0.994505 |
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Target: 5'- gCGCCGuagcggaaccAUUCGUCGG--CGUCGCgGg -3' miRNA: 3'- gGCGGC----------UAAGUAGCCuaGCAGCGaCa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 201788 | 0.66 | 0.994505 |
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Target: 5'- gCGCCGuagcggaaccAUUCGUCGG--CGUCGCgGg -3' miRNA: 3'- gGCGGC----------UAAGUAGCCuaGCAGCGaCa -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 137071 | 0.66 | 0.994505 |
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Target: 5'- aCGCCGGggUCGUCGGcgggcagCGUgaccgugagccCGCUGUc -3' miRNA: 3'- gGCGGCUa-AGUAGCCua-----GCA-----------GCGACA- -5' |
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| 13954 | 3' | -53.3 | NC_003521.1 | + | 204557 | 0.66 | 0.995237 |
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Target: 5'- cCCGCCGG-UCGUCGGcaugccggucauGUCGagCGCcGUc -3' miRNA: 3'- -GGCGGCUaAGUAGCC------------UAGCa-GCGaCA- -5' |
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Note:
When operating RNAhybrid, the pipeline first calculates the perfect minimum free energy (Perfect mfe *) of a putative miRNA when the entire putative miRNA binds to a perfectly complementary target site, then it calculates the minimum free energy of RNA duplex (mfe of the miRNA/mRNA duplex), abbreviated as Rd_mfe. An alignment for which the Rd_mfe to its correspondent Perfect mfe ratio (R_P_ratio #) is more than 66% is regarded as a positive alignment as described by Krek et al. (21). The P_value is calculated by RNAhybrid.
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